نتایج جستجو برای: brdu

تعداد نتایج: 3378  

2015
Masao Nakasatomi Akito Maeshima Keiichiro Mishima Hidekazu Ikeuchi Toru Sakairi Yoriaki Kaneko Keiju Hiromura Yoshihisa Nojima

BACKGROUND The process of epithelial-mesenchymal transition (EMT), which is generally defined by phenotypic changes of injured tubules such as loss of epithelial markers or acquisition of mesenchymal markers, implies various activating steps, including proliferation, migration, and ability to produce extracellular matrix proteins. We established here a novel approach for the detection of tubula...

Journal: :Journal of neuroscience methods 2002
N Kee S Sivalingam R Boonstra J M Wojtowicz

Adult animals continue to produce new neurons in the dentate gyrus of hippocampus. Until now, the principal method of studying neurogenesis has been to inject either tritiated thymidine or 5'-Bromo-2-deoxyuridine (BrdU) intraperitoneally followed by autoradiographic or immunohistochemical detection methods respectively. However, such exogenous markers may produce toxic effects. Our objective wa...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 1999
B Kolb B Pedersen M Ballermann R Gibb I Q Whishaw

The mitotic marker 5-bromodeoxyuridine (BrdU) was injected twice daily (60 mg/kg) into pregnant hooded rats on one of embryonic days (E) 11, 12, 13, 15, 17, or 21, or into rat pups on postnatal day (P) 10. The principal findings were the following: (1) BrdU exposure on E11 produces profound effects on body morphology, and animals must be fed a special diet because of chronic tooth abnormalities...

2006

The sister chromatid exchange (SCE) test is a widely used method to detect chemically induced genetic damage. This method has been applied in some aquatic species to monitor genetic pollutants in estuarine and marine environments. Dose response to 5-bromodeoxyuridine (BrdU) was investigated in the mussel Mytilus gallopror~incialis Lmk. Experimental assays were carried out by incorporating 4 , 1...

2003
L. M. Calvi G. B. Adams K. W. Weibrecht J. M. Weber D. P. Olson M. C. Knight R. P. Martin E. Schipani P. Divieti F. R. Bringhurst L. A. Milner H. M. Kronenberg D. T. Scadden

The procedures for bone and bone marrow section preparation, immunostaining conditions and antibodies are described in Supplementary Methods. The procedure for BrdU pulse labeling, LTR and subsequent detection has been reported. The mice were fed BrdU (0.8 mg ml in water) for 10 days, during which time 40% of LT-HSCs would divide at least once. Seventy days after BrdU labelling, sections were s...

2007
Marco Fiore Luigi Aloe Christel Westenbroek Tiziana Amendola Alessia Antonelli Jakob Korf

Rats prenatally exposed to the neurotoxins methylazoxymethanol (MAM) or 5-Bromo-2 0-deoxyuridine (BrdU) are used as animal models of brain maldevelopment. We administered in rats MAM (20 mg/kg), or BrdU (100 mg/kg) or both at gestational day 11. Locomotion was not affected by any prenatal treatment whereas learning was delayed in the Morris maze in MAM animals. BrdU induced decreased NGF and BD...

2010
Tricia H. Burdo Caroline Soulas Krystyna Orzechowski Jessica Button Anitha Krishnan Chie Sugimoto Xavier Alvarez Marcelo J. Kuroda Kenneth C. Williams

Cells of the myeloid lineage are significant targets for human immunodeficiency virus (HIV) in humans and simian immunodeficiency virus (SIV) in monkeys. Monocytes play critical roles in innate and adaptive immunity during inflammation. We hypothesize that specific subsets of monocytes expand with AIDS and drive central nervous system (CNS) disease. Additionally, there may be expansion of cells...

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