Vertical polyacrylamide gel electrophoresis (PAGE) is routinely used in molecular biology to separate macromolecules. DNA sequencing, singlestranded conformational polymorphism (SSCP) analysis and microsatellite typing all utilize the high resolution capabilities of polyacrylamide gels to separate molecules that differ in size by as little as one base pair. However, preparation of these gels is...

Biological hydrogels consisting of self-assembling peptide nanofibers are potentially excellent materials for various controlled molecular release applications. The individual nanofiber consists of ionic self-complementary peptides with 16 amino acids (RADA16, Ac-RADARADARADARADA-CONH(2)) that are characterized by a stable beta-sheet structure and undergo self-assembly into hydrogels containing...

Merrifield's solid-phase peptide synthesis'r' requires almost quantitative acylation of amino groups upon the peptidyl resin. Several methods of monitoring the course and completion of this reaction have been described (for a review see e.g,"), the ninhydrin test being the one most commonly used'. Recently, we have employed an indicative amount of 3,4-dihydro-3-hydroxy-4-oxo-I,2,3-benzotriazine...

Decolorization of six synthetic dyes using three sources of fungal laccase with the origin of Aspergillus oryzae, Trametes versicolor, and Paraconiothyrium variabile was investigated. Among them, the enzyme from P. variabile was the most efficient which decolorized bromophenol blue (100%), commassie brilliant blue (91%), panseu-S (56%), Rimazol brilliant blue R (RBBR; 47%), Congo red (18.5%), a...

Two simple, rapid and inexpensive methods based on spectrophotometry and turbidimetry have been developed for the determination of cyproheptadine hydrochloride (CPH) in pure form and in dosage forms with Bromophenol Blue (BPB) as the reagent. The spectrophotometric method is based on the formation of an ion-pair complex between the drug and BPB in an acidic buffer. The ion-pair complex formed, ...