The Brucella abortus S19 vjbR Live Vaccine Candidate Is Safer than S19 and Confers Protection against Wild-Type Challenge in BALB/c Mice When Delivered in a Sustained-Release Vehicle A. M. Arenas-Gamboa,* T. A. Ficht, M. M. Kahl-McDonagh, G. Gomez, and A. C. Rice-Ficht Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, and Department of Cellular and Molecular M...

BACKGROUND Brucellosis is a widespread zoonotic disease caused by Gram-negative Brucella bacteria. Immunisation with attenuated vaccine is an effective method of prevention, but it can interfere with diagnosis. Live, attenuated Brucella abortus strain 104M has been used for the prevention of human brucellosis in China since 1965. However, at present, no fast and reliable method exists that can ...

We previously demonstrated that a spontaneous smooth small-colony variant of Brucella abortus S19 is characterized by increased in vivo persistence and the differential expression of a gene predicted to encode a galactoside transport ATP binding protein (mglA). In order to further investigate the role of this gene in the context of its flanking regions, we analyzed the respective DNA sequences ...

Background: The intrinsic heterogeneity determination in Brucella Lipopolysaccharide (LPS) is important for explaining its chemicalnature and biological behavior. This is significant for practical purposes, since LPS is the most relevant antigen during infectionand vaccination.Objectives: The purpose of the present study was to compare biochemical and immunological differences of LPS and lipid ...

The use of liposome as an adjuvant and a vaccine carrier has been cited previously in the literature. It has also been shown to be effective in enhancing the immunogenicity of vaccine candidates. BALB/c mice immunized subcutaneously with outer membrane protein (OMP) of Brucella abortus S19 vaccine strain entrapped in a commercial cationic liposome (S19-OMP-liposome) for vaccine delivery, showed...

In the present study, we determined the levels of cytokines produced by camel (Camelus bactrianus) peripheral blood mononuclear cells (PBMCs) in response to live attenuated Brucella abortus (B. abortus) S19 vaccine. Seven camels were vaccinated with commercial B. abortus S19 vaccine, and their cytokine responses were determined using a real-time PCR assay. Cytokine responses to B. abortus S19 w...

AIM Brucellosis is a major bacterial zoonosis of global importance affecting a range of animal species and man worldwide. It has economic, public health, and bio-risk importance. Control and prevention of animal brucellosis mainly depend on accurate diagnostic tools and implementation of effective and safe animal vaccination program. There are three types of animal Brucella live vaccines - Bruc...

background: adjuvants are used to increase the immunogenicity of new generation vaccines, especially those based on recombinant proteins. despite immunostimulatory properties, the use of bacterial lipopolysaccharide (lps) as an adjuvant has been hampered due to its toxicity and pyrogenicity. brucella abortus lps is less toxic and has no pyrogenic properties compared to lps from other gram negat...

The aim of the present study was to determine comparatively the presence of anti-Brucella antibody and Brucella DNA in cow milk. Anti-Brucella antibody was detected by ELISA based on the lipopolysaccharide (LPS) as diagnostic antigen. Besides, the presence of Brucella DNA in milk samples was screened by eryCD gene-targeted PCR and B. abortus DNA was determined by amplification of alkB genes. Fo...

Brucellosis is an important zoonotic disease of nearly worldwide distribution. Despite the availability of live vaccine strains for bovine (S19 and RB51) and (Rev-1) for small ruminants but they have several drawbacks including residual virulence. So, in this study, gamma irradiated Brucella abortus vaccine prepared from an Egyptian field isolate was evaluated as a safe, stable and protective v...