We have investigated allosteric interactions of four closely related strychnine-like substances: Wieland-Gumlich aldehyde (WGA), propargyl Wieland-Gumlich aldehyde, strychnine, and brucine with N-methylscopolamine (NMS) on M(3) subtype of muscarinic receptor genetically modified in the second or the third extracellular loop to corresponding loops of M(2) subtype (M(3)o2 and M(3)o3 chimera). The...

Strychnos wallichiana (Loganiaceae) is a critically endangered medicinal plant, commonly used in several native medical practices. The majority of members of the Strychnos genus shares a similar chemical composition and contains common indole alkaloids. Purification of strychnine and brucine from a few species of Strychnos have already been reported; however, we have purified and quantified the...

The proto-oncogene c-Myb plays an important role in cell proliferation, and its upregulation affects the development of glioblastomas. G-quadruplexes are secondary DNA or RNA structures that usually form promoter region oncogenes, including c-Myb, regulate expression these genes. traditional Chinese medicine, brucine, is a ligand located c-Myb. present study investigated therapeutic effects mec...

Semen Strychni have been shown to have therapeutic effect in improving blood circulation, relieving rheumatic pain, and treating cancer. However, Semen Strychni could cause severe nephrotoxicity. The present study was designed to evaluate whether treatment with total glucosides from paeony (TGP) has renoprotective effect against nephrotoxicity induced by total alkaloids from Semen Strychni (TAS...

2014 X-ray diffraction experiments with lyotropic calamitic-nematic phases and induced lyotropic calamitic-cholesteric phases doped with brucine sulfate and d-octanol are performed. The diffraction pattern of the usual oriented nematic phase is compared with the patterns of the untwisted cholesteric phase. The analysis of the patterns indicate that even in the cholesteric phase the pseudo-lamel...

The aim of the study was to investigate the possibility of improving the therapeutic efficacy of the total alkaloid fraction (TAF) extracted from processed nux vomica by reducing the strychnine contents. Most strychnine was removed from TAF to obtain the modified total alkaloid fraction (MTAF). The toxicity and pharmacokinetics of TAF and MTAF were further investigated and compared besides thei...

A modified and highly sensitive spectrophotometric method for the determination of nitrate in trace quantities in environmental samples is described. The method is based on the reaction of nitrate ion with brucine and 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) in sulfuric acid medium to yield a violet-colored product which is stable for over two days. The optimum photometric ra...

Brucine. 2.3-Dimethoxystrychnidin-10-one was extracted from the seeds of Strychnos nux-vemica. L as described earlier (3,7). The crude alkaloid was crystallized from ethyl alcohol. melting at 178°C, The iso'lated cockroach heart technique described by Krijgsman et at. (2) was employed for the present studies. In all the experiments only adult insects were used. Sexual differences and age factor...