Background: Nicotine is the most used toxic substance in spite of mass media awareness. Column estimation nicotine found to be inhibiting factor for its estimation.Objective: To develop a method on C8 column HPLC.Methods: The involved model Waters 1515 binary HPLC Pump system interfaced with 2489 UV/VIS detector, 4.6 X 250 mm, 5μm beads Symmetry at 37°C, and an isocratic mobile phase containing...

A simple, precise, and accurate isocratic reversed-phase (RP) stability-indicating HPLC assay method was developed and validated for determination of Aripiprazole in bulk and solid pharmaceutical dosage form. A reversed-phase C8 (250×4.0 mm, 5 μm particle size) column for HPLC and C8 (50×2.1mm, 1.7 μm particle size) for UPLC method in isocratic mode was used. The mobile phase consists of aceton...

An uncontrolled used of antibiotics and sulphonamides is responsible for the presence of residues in beehive products and contributes to the problem of food safety. The optimisation of analytical methods for the detection of residues of antibacterial drugs (sulphonamides, SPs; tylosin, TL; streptomycin, ST) in honey was pursued and the methods applied to routine samples. ST was investigated by ...

A new chromatographic procedure was proposed for the separation of propranolol (PRN) enantiomers based upon enantioselective chiral ligand-exchange chromatography. The separation was carried out on a short C8 column leading to considerably short separation time. L-alanine and Cu2+ were applied as chiral selector and central bivalent complexing ion, respectively. It was found that the kind of co...

Objective: The objective of the study was to develop and evaluate the reverse phase ultra-performance liquid chromatography (RP-UPLC) method for the quantitative determination of potential impurities of rivaroxaban active pharmaceutical ingredient. Method: The method uses a water acquity BEH C8 column (100mm x 2.1 mm, 1.7μm) with mobile phase A consisted, 0.05M diammonium hydrogen phosphate, pH...

This paper describes the separation and detection of five inorganic anions (I - , BrO3 NO2 Br NO3 -) by capillary ion chromatography using 1-alkyl-3-methylimidazolium-coated columns UV at 210 nm. The salts imidazolium (IM) ions (C2-, C4-, C6-, C8-, C10-, C18-IMs) are a family ionic liquids were newly examined as permanent coating reagents on triacontyl (C30) -bonded silica reversed phase column...

Poor peak shape Wrong silica type Use type B silica Blocked frit or column void Replace frit, backflush column Silanol interactions Use amine additives, change pH, use end-capped stationary phase Excessive peak width Bad column Replace column Column overload Reduce injection volume or mass High molecular weight Normal Unresolved peaks Improve separation Inadequate retention Mobile phase too str...

Directional crystallization from the melt has been used as a tool to grow parallel crystalline stripes of p- and n-type molecular semiconductors. To start, phase behavior 2,7-dioctyl[1]benzothieno[3,2-b][1]benzothiophene (C8-BTBT-C8): tetracyanoquinodimethane (TCNQ) blends (molar ratio 20:1 1:1) investigated by variable-temperature X-ray diffraction, differential scanning calorimetry, polarized...