Present study has comparatively investigated the effect of zinc oxide nanoparticles (ZnO NPs), ZnO bulk particles (BPs), and relevant metal ions (Zn2+) on the tissue culture of rapeseed (Brassica napus L.) to properly evaluate the impact of ZnO NPs on callus induction. For finding the best hormonal combination for inducing callus, hypocotyl explants were cultured on Murashige ...

Intra- and interspecific differences in cytokinin requirement were detected in callus cultures of Phaseolus vulgaris L. and P. lunatus L. Of the ten genotypes of P. vulgaris tested in the present study, one required cytokinin for callus growth, six exhibited some to moderate growth on cytokinin-free medium, and the remaining three grew uniformly in the absence of cytokinin. In contrast, six of ...

The production of kencur (Kaempferia galanga L.) with tissue culture is an alternative in plant cultivation as a provider seeds. In culture, growth regulators can affect the formation organs (organogenesis). combination NAA and TDZ was used this study to induce callus organogenesis. purpose research determine effect giving on organogenesis callus, well optimal two callus. design factorial RAL f...

1. The polysaccharide compositions of the cell walls of sycamore cambium and sycamore callus tissue have been analysed and found to be directly comparable. 2. Electrophoretic analyses of the whole pectins prepared from actively growing callus and cambial tissue have shown that these preparations contain, in addition to the neutral and weakly acidic components present in apple fruit, a strongly ...

The metabolism of 11-14C12,4-dichlorophenoxyacetic acid (2,4-D) in soybean ( Glycine max IL.1 Merrill var. Amsoy) root callus and in differentiated soybean root cultures was investigated as a function of pesticide concentration and age of tissue. The chronological age of the tissue was found to be correlated with the mitotic index which reached a peak at 2 weeks and then declined. The metabolis...

Comparisons have been made between a sycamore callus (S4) isolated in 1970 and one first isolated in 1958 (S2). Incubation of S4 on media containing different concentrations of auxin and kinetin showed that the ratio of the hormones was the factor which controlled the growth pattern within the tissue. High ratios of auxin: cytokinin favoured a white friable tissue, whereas a compact callus with...

In tissue culture, the formation of callus from detached explants is a key step in plant regeneration; however, the regenerative abilities in different species are variable. While nearly all parts of organs of the dicot Arabidopsis thaliana are ready for callus formation, mature regions of organs in monocot rice (Oryza sativa) and other cereals are extremely unresponsive to tissue culture. Whet...

Genetic improvement of garlic through conventional breeding is very difficult due to sterile nature of its flower. Hence, an alternative system is desirable to induce genetic variation. Tissue culture could be a good opportunities and somatic embryogenesis is one of the potential techniques of tissue culture for in vitro regeneration of garlic plant. The successes and production of somatic embr...

Ultrasound is useful in demonstrating bone mineral density of regenerating osseous tissue as well as structural alterations. A proposed ultrasound method, which included ultrasonography and acoustic parameters measurement, was employed to evaluate its efficacy in monitoring the bone callus changes in a rabbit tibial distraction osteogenesis (DO) model. The findings demonstrated that ultrasonogr...

In the plant regeneration process, a great deal of variability is observable between different varieties and genotypes. Even within the same genotype, there are differences in the intensity of this process. New biologically active substances are currently being tested to increase the effectiveness of regeneration in plants. Among them are zearelonene (ZEN) – a substance obtained from an extract...