The killer toxin from Pichia membranifaciens CYC 1106, a yeast isolated from fermenting olive brines, binds primarily to the (136)-b-D-glucan of the cell wall of a sensitive yeast (Candida boidinii IGC 3430). The (136)-b-D-glucan was purified from cell walls of C. boidinii by alkali and hot-acetic acid extraction, a procedure which solubilizes glucans. The major fraction of receptor activity re...

The effect of using mixed cultures of non-Saccharomyces and Saccharomyces cerevisiae yeasts in the physicochemical and sensory qualities of the wines were analyzed in this study. Based on growth curves, sugar consumption and glycerol production in synthetic must, Candida membranifaciens L1805 was selected from a group of four Candidas spp. isolates from Chile and Argentina. This yeast was subse...

INTRODUCTION Fungemia corresponds to the isolation of fungi in the bloodstream and occurs mostly in immunosuppressed patients. The early diagnosis and treatment of these infections are relevant given the serious threat to the affected patients and possible spread to other organs, often becoming fatal. The growing number of fungemia associated with poor prognosis resulted in this research aiming...

The aim of our study was to determine species biodiversity of yeasts and yeast-like fungi in the stratum of surface water of Lake Tyrsko, as well as to evaluate the condition of waters of that lake based on the isolated species of fungi. The study was carried out from June 2007 to June 2008. The isolation and determination of the number of fungal colonies were conducted with the use of the memb...

The killer toxin from Pichia membranifaciens CYC 1106, a yeast isolated from fermenting olive brines, binds primarily to the (1-->6)-beta-D-glucan of the cell wall of a sensitive yeast (Candida boidinii IGC 3430). The (1-->6)-beta-D-glucan was purified from cell walls of C. boidinii by alkali and hot-acetic acid extraction, a procedure which solubilizes glucans. The major fraction of receptor a...

Pichia membranifaciens CYC 1086 secretes a killer toxin (PMKT2) that is inhibitory to a variety of spoilage yeasts and fungi of agronomical interest. The killer toxin in the culture supernatant was concentrated by ultrafiltration and purified to homogeneity by two successive steps, including native electrophoresis and HPLC gel filtration. Biochemical characterization of the toxin showed it to b...