Prediction of carbohydrate fractions using equations from the Cornell Net Carbohydrate and Protein System (CNCPS) is a valuable tool to assess the nutritional value of forages. In this paper, these carbohydrate fractions were predicted using data from three sunflower (Helianthus annuus L.) cultivars, fresh or as silage. The CNCPS equations for fractions B2 and C include measurement of ash and p...

Gaucher spleen sphingolipid activator protein 2 was fractionated into concanavalin A binding- and non-binding fractions. These fractions each contained several bands on non-denaturing polyacrylamide gel electrophoresis (PAGE). The two fractions were further fractionated by electroblotting the proteins from preparative gels onto nitrocellulose, staining with Ponceau S to locate the bands of prot...

The objectives of this trial were to develop multiple linear regression (MLR) models and three-layer Levenberg-Marquardt back propagation (BP3) neural network models using the Cornell Net Carbohydrate and Protein System (CNCPS) carbohydrate fractions as dietary variables for predicting in vitro rumen volatile fatty acid (VFA) production and further compare MLR and BP3 models. Two datasets were ...

Both ribonucleic and desoxyribonucleic acids have been obtained from purified particles of PR8 influenza virus. These particles were also found by extraction with formamide to contain carbohydrate in addition to that of the nucleic acids. Carbohydrate-rich fractions, essentially devoid of nucleic acid, were obtained not only from the particles representing PR8 virus but from those of Lee influe...

Normal and immune sera from various animal species were fractionated on columns of Sepharose covalently coupled with the glycoprotein fetuin. Elution of the material bound to fetuin yielded low but reproducible amounts of protein, ranging from 0.02 to 0.2% of the protein mass of the input sera. This material has been identified by immunoelectrophoresis in agar and by zone electrophoresis on cel...

The earlier resolution of preparations of bovine pancreatic deoxyribonuclease into two active fractions by chromatography on sulfoethyl-Sephadex has been improved through the use of columns of phosphocellulose. Gradient elution with sodiwn acetate at pH 4.7 shows the presence of at least four forms of the enzyme. The principal component (DNase A) is accompanied by Fractions B and C, and, in ver...

A humic acid extracted from a volcanic soil was subjected to preparative high-performance size-exclusion chromatography (HPSEC) to reduce its molecular complexity and eleven different size fractions were obtained. Cross-polarization magic-angle spinning 13C NMR (CPMAS 13C NMR) analysis performed with variable contact-time (VCT) pulse sequences showed that the largest molecular-size fractions co...

Introduction: Aloe Vera compounds have inhibitory activity on fungi, bacteria, and viruses. This study examines the antibacterial activity of A. Vera purified extracts including gel, boiled skin, boiled gel, and distilled extract against pathogenic bacteria, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Klebsiella pneumonia and Pseudomonas aeruginosa were elucidated. Method: Th...