background: mesenchymal stem cells (mscs) are multipotential cells and their therapeutic potency is under intense investigation. studying the effect of different induction factors on mscs could increase our knowledge about the differentiation potency of these cells. one of the most important sources of these factors in mammalian body is platelet. platelet lysate (pl) contains many growth factor...

Cardiomyocytes derived from human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, disease modeling, and drug discovery, all of which require enriched cardiomyocytes, ideally ones with mature phenotypes. However, current methods are typically performed in 2D environments that produce immature cardiomyocytes within heterogeneous populations. Here, we generate...

Myocardial infarction has been the leading cause of morbidity and mortality in developed countries over the past few decades. The transplantation of cardiomyocytes offers a potential method of treatment. However, cardiomyocytes are in high demand and their supply is extremely limited. Embryonic stem cells (ESCs), which have been isolated from the inner cell mass of blastocysts, can self-renew a...

Portulaca oleracea Protects H9c2 Cardiomyocytes Against Doxorubicin-Induced Toxicity by Inhibition of Oxidative Stress and Apoptosis

Five transcription factors can be used to mature stem cell-derived cardiomyocytes for better compatibility with adult cells.

Enhancers regulate gene expressions in a tissue- and pathology-specific manner by altering its activities. Plasma levels of atrial brain natriuretic peptides, encoded the Nppa Nppb, respectively, synthesized predominantly cardiomyocytes, vary depending on severity heart failure. We previously identified noncoding conserved region 9 (CR9) element as putative Nppb enhancer at 22-kb upstream from ...

Although engineered cardiac tissues (ECTs) derived from induced pluripotent stem cells (iPSCs) are promising for myocardial regenerative therapy, the appropriate ratio of cardiomyocytes to non-cardiomyocytes is not fully understood. Here, we determined whether ECT-cell content is a key determinant of its structure/function, thereby affecting ECT therapeutic potential for advanced heart failure....

Recent studies have shown that murine cardiomyocytes possess a functional intrinsic cholinergic system that can synthesize and release acetylcholine (ACh). The way by which ACh release is regulated however, is not fully understood. Classically, ACh released from neuronal sources is regulated by the vesicular ACh transporter (VAChT). We tested the hypothesis that ACh released from neonatal murin...