This study investigated the role of CBM35 from Clostridium thermocellum (CtCBM35) in polysaccharide recognition. CtCBM35 was cloned into pET28a (+) vector with an engineered His6 tag and expressed in Escherichia coli BL21 (DE3) cells. A homogenous 15 kDa protein was purified by immobilized metal ion chromatography (IMAC). Ligand binding analysis of CtCBM35 was carried out by affinity electropho...

There is a growing interest worldwide to find out new and cheap carbohydrate sources for production of bioethanol. In this work, carob pod (Ceratonia Siliqua) is proposed as an economical source for ethanol production, especially, in arid regions. The carob tree is an evergreen shrub native to the Mediterranean region, cultivated for its edible seed pods and it is currently being reemphasised a...

UNLABELLED Carob germ proteins have been shown to have functional properties similar to wheat gluten enabling formulation and production of yeast leavened gluten-free baked goods from a true dough rather than a stiff batter. The purpose of this research was to optimize the production of wheat-free bread containing carob germ flour, corn starch, NaCl, sucrose, hydroxypropyl methylcellulose (HPMC...

Carob (Ceratonia siliqua L.) pod is a characteristic fruit from the Mediterranean regions. It composed by seeds, valuable part due to extraction of locust bean gum, and pulp, considered by-product processing industry. pulp mixture macro- micronutrients, such as carbohydrates, vitamins minerals, secondary metabolites with functional properties. In last few years, numerous studies on chemical bio...

In this experiment the effect of dietary carob pods in the growth performance of fattening pigs and their meat quality, including steak chemical composition and fatty acid profile, were examined. A total of 160 weaning piglets, 30 days old, were allocated into four equal groups with 4 subgroups of 5 female and 5 males each. The animals were fed with isocaloric and isonitrogenous diets, containi...

in the course of study on larval parasitoids of carob moth, ectomyelois ceratoniae zeller (lep.: pyralidae), an hyperparasitoid wasp, pachycrepoideus vindemmiae rondani, was collected from pupae of apanteles sp. (hym.: braconidae). the latter species is the larval parasitoid of the carob moth. the p. vindemmiae belongs to the family pteromalidae and is a new record for iran insect fauna.

The present work studied low-cost carbon sources for carotenoid and lipid production using the yeast Rhodosporidum toruloides NCYC 921. Carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in R. toruloides batch cultivations. Carob pulp syrup containing a total sugar concentration of 75 g L(-1) induced the highest total fatty acid productivity...

The possibility of utilising chopped and deseeded carob pods (kibbles) as a source of polyphenolic antioxidants was examined by performing extractions with various solvent systems, in order to evaluate and optimize the conditions for the recovery of polyphenols. Maximum quantities of polyphenolic components were found in 80 % acetone extracts, as evaluated by measuring total polyphenol and tota...

BACKGROUND AND AIMS Seeds of carob, Chinese senna, date and fenugreek are hard due to thickened endosperm cell walls containing mannan polymers. How the radicle is able penetrate these thickened walls to complete seed germination is not clearly understood. The objective of this study was to determine if radicle emergence is related to the production of endo-beta-mannanase to weaken the mannan-r...