Healing As Revealed by In Vivo Imaging and Micro-CT analysis Li, X; Moore, D, Zhou FH; Robbins A; +Chen,Q +Brown University/Rhode Island Hospital, Providence, RI Jilin University, Changchun, Jilin Province, China [email protected],[email protected] INTRODUCTION: Matrilin-1(MATN1) is a cartilage specific, non-collagenous extracellular matrix protein produced by pre-hypertrophic chondrocytes in ...

Thrombospondin-2 (TSP2) is a matricellular protein with increased expression during growth and regeneration. TSP2-null mice show accelerated dermal wound healing and enhanced bone formation. We hypothesized that bone regeneration would be enhanced in the absence of TSP2. Closed, semistabilized transverse fractures were created in the tibias of wildtype (WT) and TSP2-null mice. The fractures wer...

Secondary cartilage forms on avian and mammalian dermal bones, both during normal ontogeny and during repair of fractures, but it has not been observed in any other vertebrate class. We fractured the left lower jaws of adult spotted salamanders, Ambystoma maculatum, to see whether secondary cartilage would form during fracture repair. It did not. Instead, periosteal hyperplasia produced a callu...

Objective- Current trends emphasize the acceleration of fracture healing on the ground that in doing so, the limitation of mobility and complications associated with recovery period are reduced. The present study aims to compare autogenic costal cartilage with Chitosan scaffold in canine humeral defect healing. Design- Experimental study Animal-15 adult male dogs Procedures-...

Several million people suffer from joint cartilage damage with very debilitating conditions. Once damaged, cartilage is a tissue that does not restore itself. Techniques such as micro-fracture, autogenous or allogenous osteochondral transplantation, and autologous chondrocyte implantation may be done to repair cartilage and relieve the patients’ symptoms. The environmental factors’ effect on th...

Mice are extensively used for experimental bone-healing studies. However, there are few established nondestructive in vivo techniques for longitudinal fracture-healing analysis in mice, including in vivo micro-computed tomography (μCT) and radiography. Importantly, none of the established methods can discriminate between non-mineralized fibrous tissue and cartilage in the soft fracture callus. ...

The aim of the present study was to analyze the mechanism by which nerve growth factor (NGF) promotes callus formation in mice with tibial fracture. NGF transgenic homozygotic mice and NGF wild homozygotic mice were selected to construct non-stabilized fracture model of tibia. The mice were sacrificed on days 7, 14 and 21, respectively, and each group had a sample with 8 mice at each point in t...