The quantification of allergens in food including baked food matrices is of great interest. The aim of the present study was to describe a non-immunologic method to quantify bovine β-casein using ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-TQ-MS/MS) in multiple reaction monitoring (MRM) mode. Eight of 10 theoretical peptides from β-casein after trypt...

A new liquid chromatography/mass spectrometry (LC/MS) method is described for relative quantification of phosphoproteins to simultaneously compare the phosphorylation status of proteins under two different conditions. Quantification was achieved by beta-elimination of phosphate from phospho-Ser/Thr followed by Micheal addition of ethanethiol and/or ethane-d(5)-thiol selectively at the vinyl moi...

Abstract The Toll-like receptor (TLR) signaling pathway is crucial for the initiation of effective immune responses. Computational modeling biological pathways can test mechanistic hypotheses about how regulation achieved and why it sometimes fails, causing disease (e.g., sepsis). In this investigation, mass spectrometry (LC-MS) computational are being integrated to develop a model TLR4 pathway...

The peptide hormone, prolactin, when added to organ explants of rat mammary gland, rapidly (within 1 h) induced the accumulation of casein mRNA. Casein mRNA sequences, as determined by hybridization with a specific cDNA probe, were shown to increase for up to 48 h after prolactin addition. The magnitude of this response was dependent upon the day of pregnancy at which the tissue was placed in c...

The beta-casein locus with different allelic forms of A1 and A2 has been reported among many breeds of dairy cattle. These allelic forms produce beta-casein variants of A1 and A2. The bioactive peptide of beta-casomorphin-7 is produced from proteolysis of beta-casein A1 variant. Beta-casomorphin-7 has morphine like activity and results in some diseases including type 1 diabetes mellitus, cardio...

Milk is an important component of a balanced diet and contains many valuable constituents. A considerable part of the milk healthbenefits is related to its proteins, not only for their nutritive value but also for their biological properties. Scientific evidence suggests that anticarcinogenic activities, antihypertensive properties, immune system modulation, and other metabolic features of milk...

This work evaluated the angiotensin-converting-enzyme (ACE)-inhibitory activities of a bovine sodium caseinate fermentate generated using the proteolytic capabilities of the porcine small intestinal isolate Lactobacillus animalis DPC6134 (NCIMB deposit 41355). The crude 10-kDa L. animalis DPC6134 fermentate exhibited ACE-inhibitory activity of 85.51% (+/-15%) and had a 50% inhibitory concentrat...

The action of human plasma factor XIIIa (thrombin-activated blood coagulation factor XIII) and guinea pig liver transglutaminase on purified caseins, fibrin, the derivatized gamma chain of fibrin, and a number of synthetic glutamine peptides, and peptide derivatives is reported. There are wide variations in the properties of the individual proteins and peptides as substrates for amine incorpora...

A new type of cow’s milk, called A2 has appeared in the dairy aisles supermarkets recent years. Cows’ milk generally contains two major types beta-casein as A1 and types, although there are 13 genetic variants ?-casein: A1, A2, A3, A4, B, C, D, E, F, H1, H2, I G. Studies have shown that ?-casein may be harmful, is a safer choice for human health especially infant nutrition health. The cow repor...