نتایج جستجو برای: cdna aflp
تعداد نتایج: 44127 فیلتر نتایج به سال:
Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA br...
Global gene expression profiling (GGEP) plays a pivotal role in biological research. We developed an improved GGEP method called "robust ordered mRNA differential display (RoDD)" by combining mRNA differential display (DD) and complementary DNA amplified fragment length polymorphisms (cDNA-AFLP) using elaborately designed primers and a poly (dT:A) replacement technique. Redundancy was minimized...
BACKGROUND cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) is a commonly used technique for genome-wide expression analysis that does not require prior sequence knowledge. Typically, quantitative expression data and sequence information are obtained for a large number of differentially expressed gene tags. However, most of the gene tags do not correspond to full-length (FL) coding seque...
A better understanding of virus resistance mechanisms can offer more effective strategies to control virus diseases. Papaya ringspot virus (PRSV), Potyviridae, causes severe economical losses in papaya and cucurbit production worldwide. However, no resistance gene against PRSV has been identified to date. This study aimed to identify candidate PRSV resistance genes using cDNA-AFLP analysis and ...
The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies. The aim of this study was to explore the in situ gene expression of the feca...
گلرنگ یکی از مهم ترین گیاهان دانه روغنی جهان است. عامل مرگ گیاهچه ناشی از پیتیوم یکی از مخرب ترین بیماری های گلرنگ می باشد. هدف از این مطالعه جداسازی، همسانه سازی، توالی یابی و تعیین خصوصیت ژن های درگیر در پاسخ به آلودگی پیتیوم در گلرنگ با استفاده از روش cdna-aflp بود. برای این منظور از گیاهچه های آلوده پس از آلودگی نمونه برداری گردید و rna کل استخراج شد. پس از تخلیص mrna، رشته اول و دوم cdna ...
SUMMARY A drawback of the Amplified Fragment Length Polymorphism (AFLP) fingerprinting method is the difficulty to correlate the different fragments with their DNA sequence. The AFLPinSilico application presented here simulates AFLP experiments run on either cDNA or genomic sequences, producing virtual fingerprints that allow high throughput identification of AFLP fragments. The program also en...
Analysis of differential gene expression during floral bud abortion in radish (Raphanus sativus L.).
Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain reaction ...
ارزیابی فیزیولوژیکی و مطالعه ترانسکریپتوم گیاه نخود(cicer arientinum)در واکنش به تنش خشکی انتهای فصل
تنش خشکی یکی از چالش های اصلی تولید نخود بوده و بررسی واکنش ژنوتیپ های مختلف به تغییرات رطوبت و تعیین صفات مرتبط با تحمل خشکی یکی از اجزاء اصلی برنامه های اصلاحی این گیاه به شمار می رود. این پژوهش به منظور ارزیابی فیزیولوژیک و درک بهتر اساس مولکولی تحمل خشکی در گیاه نخود تیپ کابلی به اجرا در آمد. ابتدا واکنش ژنوتیپ های مختلف در مواجهه با شیب تنش خشکی اعمال شده از طریق سیستم آبیاری بارانی تک شاخ...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید