The use of HPLC in the separation of DNA fragments is well established (1). We have adapted these techniques to the separation of cDNA from linker sequences present during lambda cloning. Short linker sequences containing an EcoRl restriction site are coupled to blunt ended cDNA, excess linkers are cleaved using EcoRl. The efficiency of cloning into the lambda EcoRl site is improved by the remo...

adenylate kinases (adk) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. in this study, the cloning of a cdna encoding an adenylate kinase from the filariaonchocerca volvulus has been described. using pcr technique, a 281 bp cdna fragment encoding part ofan adenylate kinase was isolated from an o. volvulus cdna library. use of this fragment as a p...

Here, we describe the application of a RecA-based cloning technology to generate full-length cDNA libraries enriched for genes that are differentially expressed between tumor and normal tissue samples. First, we show that the RecA-based method can be used to enrich cDNA libraries for several target genes in a single reaction. Then, we demonstrate that this method can be extended to enrich a cDN...

introduction: at present, most recombinant proteins are produced in prokaryotes especially e coli. yeasts and cho also are used as eukaryotic hosts. leishmania tarentolae, a parasite of lizards, a member of trypanosomatidae family is one of the new systems for expression of heterologous proteins. in this system, some of the parasitic protozoa features are used in expression of mammalian protein...

Research Objective A researcher used differential cDNA cloning to isolate a cDNA transcripts representing growth factor induced genes. One of these cDNA clones corresponded to a previously uncharacterized open reading frame in the human genome which the exasperated researcher named "Another Moronic Gamble" (AMG). Since increased steady-state mRNA levels could be due to either transcriptional in...

CD34 is a leukocyte antigen that is expressed in various cell types including hematopoietic cells. Monoclonal antibodies against human, murine, and canine CD34 proteins have been used for the identification of lymphohematopoietic stem/progenitor cells. The cDNA encoding bovine CD34 was cloned, and its nucleotide sequence was determined. The identity of the deduced amino acid sequence of the enc...