There is growing demand for gentle technologies to improve the lipid bioaccessibility (BA) of Chlorella vulgaris biomass while preserving cell integrity and therefore oxidative stability. Pulsed electric field treatment (PEF, 5 μs at 20 kV cm−1, 31.8 kJ kg−1sus) led an enhancement in BA from 4–7.8% (untreated) 18.7–20.9%. To reach such a level BA, incubation buffer after (12 h 25/37 °C, 48 4 °C...

Mycoparasitic Trichoderma spp. act as potent biocontrol agents against a number of plant pathogenic fungi, whereupon the mycoparasitic attack includes host recognition followed by infection structure formation and secretion of lytic enzymes and antifungal metabolites leading to the host's death. Host-derived signals are suggested to be recognized by receptors located on the mycoparasite's cell ...

OBJECTIVES In an innovative therapeutic exploitation against antibiotic-resistant Streptococcus pneumoniae, here we have evaluated the in vitro activity of a purified bacterially-encoded cell wall lytic enzyme, LytA (the major pneumococcal autolysin), and compared it with those of Cpl-1 and Pal (pneumococcal phage lytic enzymes) and two antibiotics versus four pneumococcal strains. METHODS Tw...

When washed sporulating cells of Bacillus cereus were incubated in buffer a t 37" in the presence of toluene, a partial autolysis occurred resulting in the freeing of mature and immature spores. The autolysate contained lytic enzymes which attacked vegetative cells and cell-wall preparations, releasing hexosaminecontaining peptides of characteristic constitution. The most active enzyme preparat...

An autolysin obtained from culture fluid of Staphylococcus aureus strain 8507 was purified 3,000-fold. One milligram of this preparation (S-5DL) will solubilize 12 mg of cell wall in 1 hr. The major activity is N-acetylmuramyl-l-alanine amidase. Recovery of lytic activity in the purified preparation was repeatably only 20% of the starting level. This suggests that other cell wall lytic enzymes ...

Chlamydomonas lytic enzyme of the cell wall (gamete wall-autolysin) is responsible for shedding of cell walls during mating of opposite mating-type gametes. This paper reports some topographic aspects of lytic enzyme in cells. Both vegetative and gametic cells contain the same wall lytic enzyme. The purified enzyme is a glycoprotein with an apparent molecular mass of 67 kD by gel filtration and...

Endolysins are lytic enzymes produced by bacteriophages with their ability to degrade the cell wall of bacterial hosts. Endolysin (LysABP-01) from Acinetobacter baumannii bacteriophage ØABP-01 was cloned, overexpressed and characterized. Endolysin LysABP-01 has a globular structure consisting of lysozyme-like (N-acetyl-β-D-muramidase) catalytic domain. It contains 185 amino acids which correspo...

Abstract. Putri ND, Sulistyowati L, Aini LQ, Muhibuddin A, Trianti I. 2022. Screening of endophytic fungi as potential antagonistic agents Pyricularia oryzae and evaluation their ability in producing hydrolytic enzymes. Biodiversitas 23: 1048-1057. One the most important phytopathogenic is oryzae, it causative agent rice blast diseases, which destructive detrimental disease rice. Biological con...