As obligatory intracellular pathogens, viruses exploit various cellular molecules and structures, such as cellular membranes, for their propagation. Enveloped viruses acquire lipid membranes as their outer coat through interactions with cellular membranes during morphogenesis within, and egress from, infected cells. In contrast, non-enveloped viruses typically exit cells by cell lysis, and lipi...

The mechanism of antifungal activities of propolis (Bee glue) was investigated for the growth, aflatoxins production, and lipids (total lipids, neutral lipids, phospholipids, and fatty acids) metabolism of Aspergillus parasiticus. The results of the present study indicated that propolis caused significant decrease in conidial production and conidial germination as well as mycelial growth (both ...

Nanoparticles’ uptake by cancer cells upon reaching the tumor microenvironment is often rate-limiting step in nanomedicine. Herein, we report that inclusion of aminopolycarboxylic acid conjugated lipids, such as EDTA- or DTPA-hexadecylamide lipids liposome-like porphyrin nanoparticles (PS) enhanced their intracellular 25-fold, which was attributed to these lipids’ ability fluidize cell membrane...

Cellular CD1 proteins bind lipids that differ in length (C(12-80)), including antigens that exceed the capacity of the CD1 groove. This could be accomplished by trimming lipids to a uniform length before loading or by inserting each lipid so that it penetrates the groove to a varying extent. New assays to detect antigen fragments generated within human dendritic cells showed that bacterial anti...

Ether lipids are ubiquitous constituents of cellular membranes with no discrete cell biological function assigned yet. Using fluorescent polyene-ether lipids we analyzed their intracellular distribution in living cells by microscopy. Mitochondria and the endoplasmic reticulum accumulated high amounts of ether-phosphatidylcholine and ether-phosphatidylethanolamine. Both lipids were specifically ...

the effect of epinephrine on triacylglycerol secretion was investigated in isolated rat hepatocytes. the effect appeared at concentrations of more than 1 µm and reached a plateau at 10 µm. epinephrine concentration for half of the maximal bioeffect (ec50) was about 1 µm. epineplrrine at a concentration of 10 µm suppressed triacylglycerol secretion by 33% and increased its cellular content by ap...

Neutral lipids fulfill multiple specialized roles in cellular function. These roles include energy storage and utilization, the synthesis of complex lipids in cellular membranes, lipid second messengers for cellular signaling, and the modulation of membrane molecular dynamics. We have developed a novel mass spectrometric technology, now termed shotgun lipidomics, that can identify the types and...

Lipid replacement therapy (LRT) has been used along with other strategies, such as antioxidant therapy, to replace damaged or oxidized cellular lipids that accumulate during aging and in various clinical conditions. Differing from traditional lipid nutritional supplementation, LTR replacement lipids are protected from oxidation and damage during storage, ingestion and digestion. Important lipid...

Marine microplastics pollution is a major environmental concern in marine ecosystems worldwide, yet the biological impacts of on coastal biota are not fully understood. We investigated impact suspended energy budget mussels Mytilus coruscus using Cellular Energy Allocation (CEA) approach. The were exposed to control conditions (no microplastics) or one three concentrations 2 μm polystyrene micr...

Exosomes are vesicles released by cells after fusion of multivesicular bodies with the plasma membrane. In this study, we have investigated whether ether lipids affect the release of exosomes in PC-3 cells. To increase the cellular levels of ether lipids, the ether lipid precursor hexadecylglycerol was added to cells. Lipidomic analysis showed that this compound was in fact able to double the c...