Crystalline hexokinase prepared by the method of Dar-row and Colowick consists largely of enzyme which has been modified in its chromatographic and electrophoretic behavior by yeast protease which is present during the isolation procedure. This proteolytic modification can be prevented by removal of the protease by chromatography on diethylaminoethyl cellulose; it can also be largely inhibited ...

A protease with a molecular mass of 30 kDa and the N-terminal sequence of GLQTNAPWGLARSS, was isolated from fresh fruiting bodies of the wild edible mushroom Termitomyces albuminosus. The purification protocol included ion exchange chromatography on DEAE-cellulose, Q-Sepharose, SP-Sepharose and FPLC-gel filtration on Superdex 75. The protein was unadsorbed on DEAE-cellulose and Q-Sepharose, but...

I have mapped the regions of simian virus 40 (SV40)-encoded large tumor (T) antigen that remained associated with origin-containing SV40 DNA after digestion with various concentrations of Pronase E, a nonspecific protease. Immunoaffinity-purified, labeled T antigen was bound to SV40 DNA-cellulose and treated with Pronase E. A "core" region representing amino acids 140 to about 281 was identifie...

Suberin, a major constituent of the potato periderm, is known to promote the production of thaxtomins, the key virulence factors of the common scab-causing agent Streptomyces scabiei. In the present study, we speculated that suberin affected the production of glycosyl hydrolases, such as cellulases, by S. scabiei, and demonstrated that suberin promoted glycosyl hydrolase activity when added to ...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...

conclusions in this study, we isolated b. licheniformis producing an organic solvent-stable protease from oil-contaminated soil. the protease was stable and active in various organic solvents. by purification, the protease could be used as a biocatalyst for organic solvent-based enzymatic synthesis. results according to 16srdna sequencing, strain isa9 was identified as a new strain of bacillus ...

background: fasciola hepatica is one of the most important helminthes parasites and triclabendazole (tcbz) is routinely used for treatment of infected people and animals. secreted protease enzymes by the f. hepatica plays a critical role in the inva-sion, migration, nutrition and the survival of parasite and are key targets for novel drugs and vaccines. the aim of study was to determine the pro...

Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineolytica was found to produce two proteases-P1 and P2. The proteases were purified using 60 % cold ...

Endoglucanase 2 (EG2) of the cellulolytic ruminal anaerobe Bacteroides succinogenes is a 118-kilodalton (kDa) enzyme which binds to cellulose and produces cellotetraose as the end product of hydrolysis. The purified enzyme was treated with the protease trypsin in an attempt to isolate peptides which retained the ability to either hydrolyze soluble carboxymethyl cellulose or bind to insoluble ce...

In this study, a 39-kDa metalloprotease was purified from a rare edible mushroom with health-promoting activities, Oudemansiella radicata, using a purification protocol which entailed anion exchange chromatography on DEAE-cellulose and Q-Sepharose columns and gel filtration by fast protein liquid chromatography on a Superdex 75 column. Some peptide sequences were obtained by LC-MS/MS analysis a...