We have evaluated whether selection of a human tumor leukemic line for resistance to vinblastine (Velban; VLB) alters its tumorigenicity. To address this question, CEM and CEM/VLB100 cells [which express the multiple drug-resistant (MDR) phenotype via amplification of the P-glycoprotein gene] were characterized by several techniques including chromosome banding, in situ hybridization, Southern ...

Objective(s): Biocompatibility of dental biomaterials plays a critical role in regeneration of dental stem cells. The aim of present study was to evaluate the effects of novel biomaterials of TheraCal-LC (TheraCal; Bisco), Angelus mineral trioxide aggregate (MTA; Angelus), calcium-enriched mixture (CEM; BioniqueDent), and Biodentine (Septodont) on viability of human de...

The growth-inhibitory effect of fluoropyrimidines combined with a short-term exposure to leucovorin and the pattern of polyglutamylation of folates were compared between parental CCRF-CEM cells and a cell line with impaired ability to form polyglutamates (CCRF-CEM/P). The combination of leucovorin with 5-fluorouracil or 5-fluorodeoxyuridine increased the growth inhibition of CCRF-CEM cells comp...

OBJECTIVE To determine whether overexpression of cell membrane associated drug efflux pumps belonging to the family of ATP binding cassette (ABC) proteins contributes to a diminished efficacy of sulfasalazine (SSZ) after prolonged cellular exposure to this disease modifying antirheumatic drug (DMARD). METHODS A model system of human T cells (CEM) was used to expose cells in vitro to increasin...

Glucocorticoid (GC)-evoked apoptosis of T-lymphoid cells is preceded by increases in the intracellular Ca2+ concentration ([Ca2+]i), which may contribute to apoptosis. This report demonstrates that GC-mediated upregulation of the bZIP transcriptional repressor gene, E4BP4, is dependent on [Ca2+]i levels, and correlates with GC-evoked apoptosis of GC-sensitive CEM-C7-14 cells. Calcium chelators ...

introduction: the main goal of this ex vivo study was to assess and compare the cellular and electrophysiological effects of two dental biomaterials, white mineral trioxide aggregate (wmta) and calcium enriched mixture (cem) cement, on neuronal cell excitability and electrical properties. materials and methods: a conventional intracellular current clamp technique was used to study the cellular ...

the aim of this study was to examine the effect of crude bile on the human hepg2 and ccrf-cem cell lines. cells were exposed to different dilutions of bile. antiproliferative effects were determined by the cytotoxic mtt assay. cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dutp nick end labeling (tunel) assay. bile administration resulted in dose-dependent c...

We have shown that a glycoprotein(s) with a molecular weight of approximately 180,000 exists on the surface of cultured lymphoblasts selected for resistance to vinblastine (VLB) (CEM/VLB100). The amount of this glycoprotein, which is barely detectable on the VLB-sensitive cells (CEM), appears to be related in part to the degree of resistance, up to approximately 270-fold. Exposure of the cells ...

OBJECTIVE The present study is designed to elucidate the correlation between gene dosage and increased messenger ribose nucleic acid (RNA) level in multi-drug resistant cancer cells. METHODS The human lymphoblasts CCRF-CEM (CEM) and CEM-vinblastine (VBL) 10, CEM-VBL 20, CEM-VBL 40, CEM-VBL 60, cell line CEM-VBL 80, and CEM-VBL 100 were derived from CEM VBL 10 by single step selection techniqu...

BACKGROUND Synthetic GCs serve as therapeutic agents for some lymphoid leukemias because of their ability to induce transcriptional changes via the GC receptor (GR) and trigger apoptosis. Upregulation of the BH3-only member of Bcl-2 family proteins, Bim, has been shown to be essential for GC-evoked apoptosis of leukemic lymphoblasts. Using human T cell leukemic sister clones CEM-C7-14 and CEM-C...