It was investigated whether Escherichia coli cultured in a glucose-limited chemostat is able to grow with a series of sugars whose utilization is normally repressed during batch growth with glucose. Cells growing at dilution rates of 0.2, 0.3 and 0.6h-1 were able to immediately utilize and grow with fructose, mannose, maltose and ribose. Galactose was transported instantaneously but growth star...

This is a study of high-affinity glucose uptake in Aspergillus niger and the effect of disruption of a high-affinity monosaccharide-transporter gene, mstA. The substrate saturation constant (K(s)) of a reference strain was about 15 microM in glucose-limited chemostat culture. Disruption of mstA resulted in a two- to fivefold reduction in affinity for glucose and led to expression of a low-affin...

The network structure and the metabolic fluxes in central carbon metabolism were characterized in aerobically grown cells of Saccharomyces cerevisiae. The cells were grown under both high and low glucose concentrations, i.e., either in a chemostat at steady state with a specific growth rate of 0.1 h(-1) or in a batch culture with a specific growth rate of 0.37 h(-1). Experiments were carried ou...

A phytostat to mass culture higher plant cells in liquid medium is described. This apparatus allowed the culture in batch, turbidostat and chemostat of 20 liters of cells. Automatic control of cell suspension growth was based on culture turbidity. Changes with time of certain cell characteristics, particularly cell respiration and phospholipid content, indicated that the test time to harvest la...

Shewanella loihica strain PV-4 harbors both a functional denitrification (NO3 (-)→N2) and a respiratory ammonification (NO3 (-)→NH4 (+)) pathway. Batch and chemostat experiments revealed that NO2 (-) affects pathway selection and the formation of reduced products. Strain PV-4 cells grown with NO2 (-) as the sole electron acceptor produced exclusively NH4 (+). With NO3 (-) as the electron accept...

‎In this paper‎, ‎the fractional--order form of three dimensional chemostat model with variable yields is introduced‎. ‎The stability analysis of this fractional system is discussed in detail‎. ‎In order to study the dynamic behaviours of the mentioned fractional system‎, ‎the well known nonstandard (NSFD) scheme is implemented‎. ‎The proposed NSFD scheme is compared with the forward Euler and ...