Single-use technology is now an indispensable part of biopharmaceutical production processes. This not only applies to research and development, but also commercial The manufacturers single-use systems are working on improving them for use in intensified processes as well new developments. One the bottlenecks was availability disk stack separators whose stainless steel variants widely used indu...

Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected ...

background: the critical role of interleukin-7 (il-7) in homeostatic proliferation and t cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. methods: in the present study we expressed recombinant human interleukin-7 (rhil-7) in chinese hamster ovary (cho)-k1 cells. cho-k1 cells were stably transfected wit...

The human O6-alkylguanine-DNA alkyltransferase complementary DNA was transfected into the alkyltransferase-deficient Chinese hamster ovary cell line, D422, in an effort to dissect the underlying mechanisms of bischloroethylnitrosourea (BCNU)-induced mutations. The alkyltransferase-transformed cell line exhibited 100-fold protection against BCNU-induced toxicity and an overall decrease in mutati...

Several antimitotic, tubulin-binding agents, such as colchicine, colcemid, and podophyllotoxin, inhibit the capping of fluorescent-labeled concanavalin A in Chinese hamster ovary cells. By comparing the effects of these agents on parental cell lines on several independently selected colchicine-resistant mutants with decreased drug permeability, we have demonstrated that permeation of these drug...

A Chinese hamster ovary cell mutant defective in the receptor-mediated endocytosis of several unrelated ligands (Robbins, A. R., S. S. Peng, and J. L. Marshall, 1983, J. Cell Biol., 96:1064-1071) failed to accumulate iron provided in the form of diferric transferrin. Analysis of the steps of the transferrin cycle indicated that binding and internalization of transferrin proceeded normally in mu...

Chinese hamster ovary (CHO) cells account for the production of the majority of biopharmaceutical molecules - however, the molecular basis for their versatile properties is not entirely understood yet and the underlying cellular processes need to be characterized in detail. One such process that is supposed to contribute significantly to CHO cell phenotype is methylation of DNA at cytosine resi...

Author details Department of Anesthesiology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo, Kyoto, Japan. Emergency Medicine, Kamigyo, Japan. Cardiovascular Surgery, Kyoto Prefectural University of Medicine, Kamigyo, Kyoto, Japan. Department of Anesthesiology, Kansai Medical University, Hirakata, Japan. Department of Statistical Genetics, Kyoto University, Kamigyo, ...

Mitomycin C (MMC), a bifunctional alkylating agent, requires metabolic reduction to become biologically active. We have identified a series of genetically related Chinese hamster ovary cell lines which span approximately three orders of magnitude in the concentration of MMC required for cell killing. Many mechanisms, including drug transport, drug activation, drug detoxification, and the elimin...