Peptide mapping, a key analysis used in the characterization of bio-therapeutic proteins, results in complex chromatograms that are generally difficult to study. The enzymatic treatments applied to the proteins are reproducible, but minor changes in protein structure can result in very slight changes in the quantities of protein fragments. Thus, overlaying the chromatograms in a side-by-side or...

Frequency-pulse-modulated electron capture gas-liquid chromatography was used in conjunction with appropriate derivatization procedures to obtain chromatograms from extracts of pleural effusions. These chromatograms were used to rapidly classify the various types of pleural effusions. With this method we have been able to distinguish among a limited number of effusions caused by congestive hear...

A new NMF algorithm has been proposed for the deconvolution of overlapping chromatograms of chemical mixture. Most of the NMF algorithms used so far for chromatogram separation do not converge to a stable limit point. To get same results for all the runs, instead of random initialization, three different initialization methods have been used namely, ALS-NMF (robust initialization), NNDSVD based...

Strains of saccharolytic and nonsaccharolytic Pseudomonas species were examined by a new single-step gas chromatographic characterization procedure. Cells were digested in a methanolic solution of tetramethylammonium hydroxide pentahydrate, and the digestates were subjected to gas-liquid chromatographic analysis. The chromatograms were examined for similarities and differences in their overall ...

Manual or computer assisted peak deconvolution on chromatograms of the racemate of 1-chloro-2,2-dimethylaziridine enantiomers GC was used to determine the peak areas of enantiomers in the racemate prior (AA,0, AB,0) and after the separation (AA, AB). These peak areas were used in the determination of apparent rate constants and apparent energy barrier to enantiomerization. Comparison of apparen...

Study of Band Broadening occurring in Size Exclusion Chromatography (SEC) is reported using very narrow PS standards obtained and characterised by Temperature Gradient Interaction Chromatography (TGIC). Chromatograms are fitted by Exponentially Modified Gaussian functions (EMG) and mapping of band broadening is obtained for different column sets. Interpretation of the skewing of the chromatogra...

Two-dimensional gas chromatography (GC×GC) plays a central role into the elucidation of complex samples. The automation of the identification of peak areas is of prime interest to obtain a fast and repeatable analysis of chromatograms. To determine the concentration of compounds or pseudo-compounds, templates of blobs are defined and superimposed on a reference chromatogram. The templates then ...

Analysis of accelerants in fire debris involves the isolation of residual volatiles from the matrix and the analysis of these volatiles, usually by gas chromatography (GC). The resulting chromatograms are interpreted by comparing to a library of accelerant chromatograms obtained under similar conditions. This review first mentions ASTM's system in classifying fire accelerants into light petrole...