نتایج جستجو برای: chromosomal integration

تعداد نتایج: 268172  

Journal: :Journal of clinical microbiology 2006
Katherine N Ward Hoe Nam Leong Elisabeth P Nacheva Julie Howard Claire E Atkinson Nicholas W S Davies Paul D Griffiths Duncan A Clark

Six immunocompetent patients with human herpesvirus 6 (HHV-6) chromosomal integration had HHV-6 and beta-globin DNA quantified in various samples by PCR. The mean HHV-6 DNA concentration (log(10) copies/milliliter) in blood was 7.0 (>/=1 HHV-6 DNA copies/leukocyte), and in serum it was 5.3 (>/=1 HHV-6 DNA copies/lysed cell). The mean HHV-6 DNA load (log(10) copies)/hair follicle was 4.2 (>/=1 c...

2015
Karen Roos Esther Werner Holger Loessner

Chromosomal integration of expression modules for transgenes is an important aspect for the development of novel Salmonella vectors. Mini-Tn7 transposons have been used for the insertion of one such module into the chromosomal site attTn7, present only once in most Gram-negative bacteria. However, integration of multiple mini-Tn7 copies might be suitable for expression of appropriate amounts of...

Journal: :Human gene therapy 2006
Anja Ehrhardt Jeffrey A Engler Hui Xu Athena M Cherry Mark A Kay

Reports on insertional mutagenesis due to integration of gene therapy vectors into the host genome have raised concerns about the genetic manipulation of somatic cells. Previously, it was demonstrated that integrase phiC31 derived from a Streptomyces phage mediates site-specific integration into the host genome of mammalian cells in vitro and in vivo by recombining the attB recognition site in ...

Journal: :BioTechniques 2006
Elizabeth C Bryda Michael Pearson Yuksel Agca Beth A Bauer

Transgene delivery systems, particularly those involving retroviruses, often result in the integration of multiple copies of the transgene throughout the host genome. Since site-specific silencing of trangenes can occur; it becomes important to identify the number and chromosomal location of the multiple copies of the transgenes in order to correlate inheritance of the transgene at a particular...

Journal: :Human gene therapy 2010
L Gama-Norton S Herrmann R Schucht A S Coroadinha R Löw P M Alves C C Bartholomae M Schmidt C Baum A Schambach H Hauser D Wirth

The improvement of safety and titer of retroviral vectors produced in standard retroviral packaging cell lines is hampered because production relies on uncontrollable vector integration events. The influences of chromosomal surroundings make it difficult to dissect the performance of a specific vector from the chromosomal surroundings of the respective integration site. Taking advantage of a te...

Journal: :Cancer research 1999
L A Koopman K Szuhai J D van Eendenburg V Bezrookove G G Kenter E Schuuring H Tanke G J Fleuren

Progressive chromosomal changes and integration of human papillomavirus (HPV) sequences mark the development of invasive cervical cancer. Chromosomal localization of HPV integration is essential to the study of genomic regions involved in HPV-induced pathogenesis. Yet, the available information about HPV integration loci is still limited, especially with respect to different HPV types. We have ...

Journal: :Journal of insect physiology 2003
D E Gundersen-Rindal D E Lynn

The long-term persistence of polydnavirus (PDV) DNA in infected lepidopteran cell cultures has suggested that at least some of the virus sequences become integrated permanently into the cell genome. In the current study, we provide supportive evidence of this event. Cloned libraries were prepared from two different Lymantria dispar (gypsy moth) cell lines that had been maintained in continuous ...

2013
Niels GA Kuijpers Soultana Chroumpi Tim Vos Daniel Solis-Escalante Lizanne Bosman Jack T Pronk Jean-Marc Daran Pascale Daran-Lapujade

In vivo assembly of overlapping fragments by homologous recombination in Saccharomyces cerevisiae is a powerful method to engineer large DNA constructs. Whereas most in vivo assembly methods reported to date result in circular vectors, stable integrated constructs are often preferred for metabolic engineering as they are required for large-scale industrial application. The present study explore...

2015
Mario Juhas James W Ajioka

The Gram-negative bacterium Escherichia coli is routinely used as the chassis for a variety of biotechnology and synthetic biology applications. Identification and analysis of reliable chromosomal integration and expression target loci is crucial for E. coli engineering. Chromosomal loci differ significantly in their ability to support integration and expression of the integrated genetic circui...

2011
Kristina Nehlsen Leonor da Gama-Norton Roland Schucht Hansjörg Hauser Dagmar Wirth

The strength of recombinant gene expression is a key property of cell lines for biopharmaceutical protein production. In most stable cell lines the expression vector is stably introduced into the host chromosomal DNA. Apart from the copy number and the used expression control elements the performance of recombinant expression vectors is modulated by genetic and epigenetic features provided by f...

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