Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes sev...

Citrus tristeza virus (CTV) causes two citrus diseases that have caused devastating losses in global citrus production. The first disease is quick decline of trees propagated on the sour orange rootstock. The second disease is stem pitting, which severely affects a number of economically important citrus varieties regardless of the rootstock used and results in reduced tree growth and vigor as ...

Citrus sudden death (CSD) has caused the death of approximately four million orange trees in a very important citrus region in Brazil. Although its etiology is still not completely clear, symptoms and distribution of affected plants indicate a viral disease. In a search for viruses associated with CSD, we have performed a comparative high-throughput sequencing analysis of the transcriptome and ...

Background and Aims: Plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. Reverse transcriptase-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certification of plant propagating materials. The main object of the present study was the optimization ...

The control of Citrus tristeza virus (CTV), the causal agent of tristeza disease, is essential to guarantee a productive citrus industry. The specific features of a nursery block make the control of the natural spread of viruses transmitted by vectors more difficult. Thus, the knowledge of the epidemiology of CTV in nursery blocks is basic to the design control strategies of the disease. Two ex...

gummy bark disease of sweet orange (citrus sinensis) trees has been reported from a few citrus growing regions of the world. the disease symptoms consist of mild to severe stem pitting and brown gummy flecks in the bark of sweet orange trees on sour orange ( c. aurantium) rootstock, being conspicuous several centimeter above the bud :union: on the trunk. the pits were usually impregnated with b...

LIKUBIN has been prevalent in Taiwan since 1958, attacking most of the commercial varieties such as ponkan, tankan, and sweet orange. Formerly thought to be due to unfavorable soil conditions, such as high moisture content and deficiency of essential nutrients, or to root rot, it was subsequently demonstrated to be caused by an infectious agent believed to be closely related to tristeza virus b...

ABSTRACT Citrus Huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide. The causal agent of HLB in Florida is thought to be 'Candidatus Liberibacter asiaticus'. In this work, we examined the responses of 30 different genotypes of citrus to Florida isolates of 'Ca. L. asiaticus' under controlled conditions in the greenhouse or growth room. Although 'Ca. L. asiaticus' was...

Citrus tristeza is one of the most destructive citrus diseases and is caused by the phloem-restricted Closterovirus, Citrus tristeza virus. Mild strain CTV-B2 does not cause obvious symptoms on indicators whereas severe strain CTV-B6 causes symptoms, including stem pitting, cupping, yellowing, and stiffening of leaves, and vein corking. Our laboratory has previously characterized changes in tra...

Citrus tristeza virus (CTV) is a major pathogen affecting citrus trees worldwide. However, few studies have focused on CTV's evolutionary history and geographic behavior. CTV is locally dispersed by an aphid vector and long distance dispersion due to transportation of contaminated material. With the aim to delve deeper into the CTV-NC (New Clade) genotype evolution, we estimated an evolution ra...