An ion pair reversed phase HPLC method for the determination of clavulanic acid has been developed. Since clavulanic acid had poor absorption O,mas 201 nm in water) in a UVregion suitable for HPLC detection, the detectability was enhanced by bathochromic shift of )maa due to solvent effect. The shifts of 2ma.., were measured with the solutions containing clavulanic acid, tetrabutylammonium brom...

The efficacy of an antimicrobial agent in the therapy of infection depends upon the interaction of bacteria, antibiotic and phagocytes. The influence of both ticarcillin/clavulanic acid and amoxycillin/clavulanic acid on the phagocytic and bactericidal activity of human PMNs in vitro was investigated. The results show that clavulanic acid, with its beta-lactamase inhibitory properties, potentia...

Disk diffusion susceptibility testing of ticarcillin plus the beta-lactamase inhibitor clavulanic acid was performed on 489 clinical isolates, and the results were compared with reference broth microdilution susceptibilities. Four different disks containing 75 micrograms of ticarcillin plus 2.5, 5, 10, and 15 micrograms of clavulanic acid were evaluated. Based on test performance and clavulanic...

BACKGROUND/AIM Quantitative analysis of amoxicillin and clavulanic acid in biological matrices requires sensitive and specific methods which allow determination of therapeutic concentration in 1 g/mL range. Analytical methods for determination of their concentrations in body fluids described in literature include high performance liquid chromatography coupled to UV detector (HPLC-UV) and liquid...

A model of acute Legionella pneumophila pneumonia in neutropenic weanling rats was developed as a means of assessing the efficacies in vivo of the beta-lactams ticarcillin, ticarcillin-clavulanic acid, and clavulanic acid, agents active against the organism in vitro. Weanling rats were dosed with cyclophosphamide 3 days before and immediately prior to infection by intrabronchial intubation with...

nowadays microbial biotechnology is considered as one of the most important and profitable branches of industry. in this approach, by using the generator microorganisms, we are able to produce biotechnological products, with higher efficiency. clavulanic acid is being used in combination with commonly used beta-lactam antibiotics in order to fight against bacterial infections that are resistant...

BACKGROUND The most common sequelae after surgical removal of mandibular third molar are pain, trismus, swelling, and dysphagia. However, these symptoms can also signal the onset of surgical site infection and alveoli osteitis. The aim of this study was to evaluate the efficacy of prophylactic amoxicillin/clavulanic acid and levofloxacin and preemptive therapy of amoxicillin/clavulanic acid in ...

the clar of streptomyces clavuligerus in the clavulanic acid gene cluster encodes a transcriptional regulator that controls clavulanic acid biosynthesis. the main goal of this study was isolation and molecular detection of the clar gene and its cloning in the streptomyces specific vector (pma:: hyg). by cinsideration of the clar gene’s start codon, the specific primers were designed. after geno...

In vitro, growth of Chlamydia trachomatis was not entirely eliminated by 960 micrograms of ticarcillin per ml, 64 micrograms of amoxicillin per ml, 32 micrograms of clavulanic acid per ml, a combination of ticarcillin (480 micrograms/ml) and clavulanic acid (32 micrograms/ml), and a combination of amoxicillin (32 micrograms/ml) and clavulanic acid (8 micrograms/ml). However, a greater than or e...