CLIP-170 is a cytoplasmic linker protein that localizes to plus ends of microtubules in vivo. In this study, we have characterized the microtubule-binding properties of CLIP-170, to understand the mechanism of its plus end targeting. We show that the NH2-terminal microtubule-interacting domain of CLIP-170 alone localizes to microtubule plus ends when transfected into cells. Association of CLIP-...

Extracellular proteases play an important role in a wide range of host physiological events, such as food digestion, extracellular matrix degradation, coagulation and immunity. Among the large extracellular protease family, serine proteases that contain a "paper clip"-like domain and are therefore referred to as CLIP-domain serine protease (clip-SP), have been found to be involved in unique bio...

Cytoplasmic linker protein (CLIP)-170, CLIP-115, and the dynactin subunit p150(Glued) are structurally related proteins, which associate specifically with the ends of growing microtubules (MTs). Here, we show that down-regulation of CLIP-170 by RNA interference results in a strongly reduced accumulation of dynactin at the MT tips. The NH(2) terminus of p150(Glued) binds directly to the COOH ter...

A 56-year-old woman presented with a 1-month history of right upper quadrant abdominal pain after a laparoscopic cholecystectomy for gallstones. Her medical history included endoscopic retrograde cholangiopancreatography (ERCP) performed 4months prior to the laparoscopic cholecystectomy for the treatmentof common bile duct (CBD) stones. During this procedure duodenal ulcers had also been found ...

the objective of this study was to determine the chronology of changes in blood pressure in renalhypertension induced by solid plexiglass clips in rats. saw blades with the thickness of 0.21-0.22 mm wereused to make clips sized 4 × 2 × 2 mm from a piece of 2-mm thick plexiglass. rats were subjected to shamoperation or left renal arterial clipping, and 1, 2, 3 and 4 weeks later blood pressure, a...

conclusions retrograde laparoscopic appendicectomy using single polymer clip makes easy access to operating in complicated and non-complicated appendicitis. results four patients (15.4%) were converted to open surgery; three patients in group i and one in group ii. four patients developed postoperative complications; three patients in group i and one in group ii. operative time was less in grou...

introduction:different types of prosthesis are available for stapes replacement. because there has been no published report on the efficacy of the titanium soft-clip vs the fluoroplastic causse loop teflon piston, we compared short-term hearing results of both types of prosthesis in patients who underwent stapedotomy due to otosclerosis.materials and methods:a total of 57 ears were included in ...

Several MHC class II alleles linked with autoimmune diseases form unusually low stability complexes with CLIP, leading us to hypothesize that this is an important feature contributing to autoimmune pathogenesis. To investigate cellular consequences of altering class II/CLIP affinity, we evaluated invariant chain (Ii) mutants with varying CLIP affinity for a mouse class II allele, I-E(d), which ...