The mechanism by which metalloporphyrins escape from mitochondria has been studied in isolated rat-liver mitochondria using Co-deuteroporphyrin as the model compound. During the first 10--15 min of incubation the efflux is about 10% of the total amount of Co-deuteroporphyrin synthesized. The efflux then increases to a second steady-state leve of 25--35% after 30--45 min of incubation. The efflu...

This study discusses poly(3-hydroxybutyrate-co-4-hydroxybutyrate) that is produced by Delftia acidovorans bacteria in fed-batch cultures from various carbon sources. The experimentally determined optimal fed-batch culture conditions are as follows: pH: 7.0, incubation temperature: 26 8C, incubation time: 72 h, and concentration of carbon source: 10 g/L. The maximum 4HB unit composition of P(3HB...

Abstract Study question Does a short-time co-incubation in conventional IVF improve rates of fertilization, embryo development and euploidy, when compared to overnight co-incubation? Summary answer There is no significant difference euploidy between sibling oocytes subjected short versus co-incubation. What known already Conventional usually implies coincubation cumulus-oocyte complex(es) with ...

In the dark, leaf tissue of crisphead lettuce (Lactuca sativa L.) metabolized (14)CO to (14)CO(2) and acid-stable products. Tissue incubated at 2.5 degrees C for 3.5 hours and 48 hours converted about 1% and 17%, respectively, of the applied (14)CO to (14)CO(2), and incorporated about 0.04% and 0.6% of the (14)C in acid-stable products. Examination of soluble acid-stable products from (14)CO an...

Methods: Two co-cultured strains of L. intracellularis were suspended in vitro for 30 minutes at 20°C in various disinfectants according to label recommendations for usage, then washed in saline. Disinfectanttreated and Control bacteria were then added to fresh cells for co-culture for a standard 5-day incubation. Further cell-free suspensions were exposed to air at 5°C, 20°C, or 37°C for up to...