Lauryl sulphate broth, introduced by Cowles (1938) and modified by Mallman and Darby (1941), has been widely used in recent years for the presumptive test for coliform bacteria in water. Lauryl sulphate is, to a certain extent, an inhibitory agent and tends to suppress anaerobic lactose fermenting bacteria. Inhibitory agents in culture media are always subject to suspicion. A substance which ca...

Water quality data at 12 sites within an urban, a suburban, and a rural stream were collected contemporaneously during four wet and eight dry periods. The urban stream yielded the highest biochemical oxygen demand (BOD), orthophosphate, total suspended sediment (TSS), and surfactant concentrations, while the most rural stream yielded the highest total organic carbon concentrations. Percent wate...

Genetically feathered and featherless sibling broilers selected for matched BW were killed, scalded, and defeathered to determine the consequences of feathers and empty feather follicles on the recovery of bacteria from carcass breast skin. In trial 1, the vents of all carcasses were plugged and sutured before scalding to prevent the expulsion of cloacal contents during picking. In trial 2, hal...

Fecal coliform bacteria cause water quality problems for many urban areas across the United States. The objective of this study is to determine if the Irwin Creek and Sugar Creek wastewater treatment plants, located near Charlotte in Mecklenburg County, North Carolina, contribute significantly to the fecal coliform concentrations in the creeks. Fecal coliform densities in the wastewater effluen...

The aims of this study were to determine the total population of coliform bacteria in the samples collected from diarrhea associated patients from the local area of Bangladesh and to examine the antibacterial efficacy of leaf extracts of Moringa oleifera (Moringaceae) against the isolated coliform bacteria. The coliform bacteria detected in these samples by some microbial-biochemical tests such...

Contamination of surface waters with fecal bacteria from grazinglands is a component of nonpoint source agricultural pollution. Methods are needed to limit the movement of fecal bacteria from grazinglands into surface water. We used two experimental watersheds to test for impact of cattle on total coliform, E. coli, and enterococci bacteria numbers. Grazing cattle elevated these microbe numbers...

A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of (14)CO(2) from [(14)C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved (14)CO(2) is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of ...

Four brands of membrane filters were examined for total and fecal coliform recovery performance by two experimental approaches. Using diluted EC broth cultures of water samples, Johns-Manville filters were superior to Sartorius filters for fecal coliform but equivalent for total coliform recovery. Using river water samples, Johns-Manville filters were superior to Sartorius filters for total col...