It is generally accepted that the functional compartmentalization of eukaryotic cells is reflected by the differential occurrence of proteins in their compartments. The location and physiological function of a protein are closely related; local information of a protein is thus crucial to understanding its role in biological processes. The visualization of proteins residing on intracellular stru...

We introduce a novel statistical approach that quantifies, for the first time, the amount of colocalization of two fluorescent-labeled proteins in an image automatically, removing the bias of visual interpretation. This is done by estimating simultaneously the maximum threshold of intensity for each color below which pixels do not show any statistical correlation. The sensitivity of the method ...

Gershome C, Lin E, Kashihara H, Hove-Madsen L, Tibbits GF. Colocalization of voltage-gated Na channels with the Na /Ca exchanger in rabbit cardiomyocytes during development. Am J Physiol Heart Circ Physiol 300: H300–H311, 2011. First published October 22, 2010; doi:10.1152/ajpheart.00798.2010.—Reverse-mode activity of the Na /Ca exchanger (NCX) has been previously shown to play a prominent role...

Colocalization of dihydropyridine (DHPR) and ryanodine (RyR) receptors, a key determinant of Ca(2+)-induced Ca2+ release, was previously estimated in 3-, 6-, 10-, and 20-day-old rabbit ventricular myocytes by immunocytochemistry and confocal microscopy. We now report on the effects of deconvolution (using a maximum-likelihood estimation algorithm) on the calculation of colocalization indexes. C...

Scriven DR, Lynch RM, Moore ED. Image acquisition for colocalization using optical microscopy. Am J Physiol Cell Physiol 294: C1119–C1122, 2008. First published March 19, 2008; doi:10.1152/ajpcell.00133.2008.—Colocalization, in which images of two or more fluorescent markers are overlaid, and coincidence between the probes is measured or displayed, is a common analytical tool in cell biology. I...