vorax strain V,. Serological relationships were determined by three procedures: immobilization, agglutination, and complement fixation. Antigenic relationships in general were dependent upon the method employed. The immobilization test provided the best method for uncovering these differences. The complement fixation test did not provide satisfactory identification of the different species.

using complement fixation technique, serial serum samples from 15 patients undergoing surgical removal of malignant tumors were assayed for the presence of tumors antibodies against the cellular fractions of both normal and carcinomatous tissue. antibody responses against tumor associated antigens were detected in four cases. in all cases antibody activity was directed only against the supernat...

The immunodiffusion and complement fixation tests for histoplasmosis were compared on 2,090 initial sera from 70 proven cases of histoplasmosis, 177 routine chest hospital patients, and 1,843 coal miners from outside the major endemic area for histoplasmosis. The complement fixation test using histoplasmin as antigen detected antibodies in the sera of 72.8% of the 70 proven cases, while the com...

A complement fixation test modified by the addition of porcine serum and an indirect hemagglutination test were used to detect antibodies to Pseudomonas pseudomallei in pigs. These tests together with cultural examinations were carried out with 250 pigs. The sensitivity and specificity values were 79.3 and 99.5% and 82.8 and 93.2% for the modified complement fixation and hemagglutination tests,...

False positive raised complement fixation titres to egg-grown virus preparations were found in six pigeon fanciers suffering from extrinsic allergic alveolitis but not in six similar budgerigar fanciers. The raised titres in the pigeon fanciers fell fourfold over a short interval in the absence of avian exposure and were attributable to antibodies directed against antigens from hen's egg in whi...

Modification of the Microtiter reading mirror used in the standardized diagnostic complement fixation method permits convenient estimation of the results in per cent hemolysis by direct visual comparison with the hemolytic standards.

An automated method to detect TrypanosomÐ crozi antibodies is described, using complement fixation with an adequate automated equipment. Fifty five serum samples from patients lvhere Trypanosoma cruzi antiþodies were detected by Almeida's technique for complement fixation and by fndirect Immunofluorescence test, were studied by this method. The conclusion is that this automated üechnique can be...

Paired specimens of serum from patients from whom Bacteroides fragilis had been isolated were tested by complement fixation against a crude B. fragilis antigen. A high titre or a rise in titre to B. fragilis was obtained in each of five patients with infection after abdominal surgery but in none of 11 patients with postpartum pyrexia nor in nine with vaginitis.

Antigenic relationships between 12 locally isolated bdellovibrios and 3 established reference strains (109D, 6-5-S, and UKi2) were investigated. Antigenicity of the strains was examined by use of the micro-complement fixation test, the serum and complement bactericidal test, and the immunodiffusion test. Antisera were prepared against one of the local strains (MS7) and against one of the establ...

Variables involved in micro complement fixation (CF) tests for type-specific (TSM) and non-type-specific (NTSM) antibodies against preparations of streptococcal M protein were studied. Sera of rabbits immunized with purified M protein which contained high titers of anti-TSM and low titers of anti-NTSM antibodies reacted type specifically in the CF tests when relatively low concentrations of M p...