Hepatitis delta virus (HDV) is a viroid-like blood-borne human pathogen that accompanies hepatitis B virus infection in 5% patients. HDV has been studied for four decades; however, the knowledge on its life-cycle and pathogenesis is still sparse. The studies are hampered by the absence of the commercially-available HDV-specific antibodies. Here, we describe a set of reproducible methods for the...

BACKGROUND Safe and effective treatment for chronic inflammatory and neuropathic pain remains a key unmet medical need for many patients. The recent discovery and description of the transient receptor potential family of receptors including TRPV1 and TRPA1 has provided a number of potential new therapeutic targets for treating chronic pain. Recent reports have suggested that TRPA1 may play an i...

Current human papillomavirus (HPV) major capsid protein L1 virus-like particles (VLPs)-based vaccines in clinic induce strong HPV type-specific neutralizing antibody responses. To develop pan-HPV vaccines, here, we show that the fusion protein E3R4 consisting of three repeats of HPV16 L2 aa 17-36 epitope (E3) and a modified human IgG1 Fc scaffold (R4) induces cross-neutralizing antibodies and p...

introduction: inflammation and edema symptoms are physiological response to triggers that can be induced by different mediators such as cytokines. rheumatoid arthritis is the most common form of arthritis which is characterized by chronic inflammation of the synovial membrane, severe and debilitating pain and progressive cartilage injury. it is clear that cytokines are involved in different sta...

A recent clinical trial in African children demonstrated the potential utility of merozoite surface protein (MSP)-3 as a vaccine against Plasmodium falciparum malaria. The present study evaluated the use of Plasmodium vivax MSP-3 (PvMSP-3) as a target antigen in vaccine formulations against malaria caused by P. vivax. Recombinant proteins representing MSP-3α and MSP-3β of P. vivax were expresse...

OBJECTIVE Shark type II collagen (SCII) is extracted as a glycoprotein from the cartilage of blue shark (Prionace glauca). We aim to confirm the effects of oral tolerance of SCII on inflammatory and immune responses to the ankle joint of rheumatoid-arthritis rats induced by Complete Freund's Adjuvant (CFA). MATERIALS AND METHODS The onset of rheumatoid arthritis (RA) was observed 14 ± x days ...

aviecennia marina (avicenniaceae) is an endemic plant that widely distributed in the southern parts of iran. this plant has been used as treatment of rheumatism arthritis among the inhabitants of southern parts of iran. the avicennia marina hydroalcoholic extract was prepared and its protective efficacy was investigated using measurement of ankle diameter, total wbc and rbc count, esr, and pro-...

activated macrophages have been classified into classical (m1) and alternative (m2) macrophages. we aimed to establish a method to yield enough number of macrophages to analyze their molecular, biological and immunological functions. we used drugs; adjuvant albumin from chicken egg whites—imject alum (ova- alum) and ova complete freund adjuvant (ova-cfa), to induce macrophages to m2 and m1 resp...

a field study was conducted to evaluate the effect of haloxyfop-r-methyl ester doses in different grass weeds in various growing stages in safflower during 2011-2012 growing season in research farm of college of agriculture, shiraz university. the study was carried our as split split plot based on randomized complete block design with three replicates. the main factor, sub factor and sub sub fa...