storage protein, was detected from our wild soybean germplasm collection. AItheugh induced mutant Iines lacking B-cenglycinin were reported to be non viable in preyious studies, the QT2 line grew nermally and produced successiye generations. To distinguish it from the non yiable B-conglycinin-lacking line, this phenotype was referred to as the "QT2-type". In these studies, we investigated the m...

Novokinin (RPLKPW), LPYPR, and rubiscolin (YPLDLF) are bioactive peptides with respective hypotensive, hypocholesterolemic, and memory-enhancing activities. We generated transgenic soybean lines that expressed modified forms of the alpha' subunit of seed storage protein beta-conglycinin containing tandem repeats of these bioactive peptides. The modified alpha' subunits constituted up to 0.2% of...

Title The regulatory function of the upstream sequence of the beta-conglycinin alpha subunit gene in seedspecific transcription is associated with the presence of the RY sequence Author(s) Yoshino, Michiko; Nagamatsu, Atsushi; Tsutsumi, Ken-ichi; Kanazawa, Akira Citation Genes & Genetic Systems, 81(2): 135-141 Issue Date 2006-04 Doc URL http://hdl.handle.net/2115/14641 Type article (author vers...

In this study, soy proteins were reduced with 0.1-10mM dithiothreitol (DTT) to obtain an increasing number of sulphydryl groups (SH) with a similar particle size. Aggregation was promoted by increasing the degree of reduction when heated (100°C, 30min), resulting in larger sized aggregates (from 40 to 70nm) and a higher viscosity of the aggregate dispersion. The disulphide bond (SS) content dec...

Upstream sequences of the gene encoding the a‘ subunit of 8-conglycinin were analyzed for interactions with nuclear proteins from immature soybean seeds. Two factors were identified that interact with specific sequence elements within 257 base pairs 5‘ of the transcription start site. One factor, SEF 3, binds exclusively to a region composed of two elements located at -183 to -169 base pairs an...

Seeds possess a high intrinsic capacity for protein production that makes them a desirable bioreactor platform for the manufacture of transgenic products. One strategy to enhance foreign protein production involves exchanging the capacity to produce intrinsic proteins for the capacity to produce a high level of foreign proteins. Suppression of the alpha/alpha' subunit of beta-conglycinin storag...

Various salt solutions used to hydrate full-fat cracked soybeans affected the thermal stability of both f)-conglycinin (75) and glycinin (115) storage proteins when examined by differential scanning calorimetry used to monitor both denaturation temperature (Td) and enthalpy (LiH). As salt ionic strength (fL) increased, the moisture in the hydrated beans decreased. When beans were hydrated with ...

empeh is an Indonesian traditional fermented food with rich nutrition and bioactive components. Rhizopus sp,especially oligosporus), lactic acid bacteria (Lactobacillus sp.), yeast aremicroorganisms involved in Tempeh fermentation. An interesting offer of Bioinformatics (in silico method) as a supporting tool molecular biology studies has emerged, such protein cleavage. This study utilized Pept...

Phytate-removed and deamidated soybean β-conglycinin (PrDS) prepared by ion-exchange resins was supplemented to be 4% in the diet administered to ovariectomized rats to investigate its preventive effect on osteoporosis. The apparent calcium absorption rate decreased following ovariectomy and was not replenished by oral administration of phytate-removed soybean β-conglycinin (PrS) or casein. On ...