In this research, we synthesized a novel DNA-polymer conjugate and evaluated its application to an affinity precipitation separation of TATA-box binding protein (TBP), which is a representative general transcription factor. The conjugate was composed of two fractions. One was a double-stranded DNA modified by the grafting of poly(N-isopropylacrylamide) (PNIPAAm), which is known as a thermosensi...

A fluorescent metallointercalator conjugate that selectively targets DNA base mismatches has been synthesized by coupling an organic fluorophore to a bulky Rh intercalator containing the chrysenequinone diimine ligand. Ion pairing between the cationic Rh and anionic fluorophore moieties dramatically quenches the fluorescence of the conjugate in solution and in the presence of matched DNA. Howev...

Highly diastereo- and enantioselective conjugate addition of α-substituted nitroacetates to maleimides under base-free neutral phase-transfer conditions was developed for the synthesis of α,α-disubstituted α-amino acid derivatives.

Generalized linear mixed models with semiparametric random effects are useful in a wide variety of Bayesian applications. When the random effects arise from a mixture of Dirichlet process (MDP) model with normal base measure, Gibbs sampling algorithms based on the Pólya urn scheme are often used to simulate posterior draws in conjugate models (essentially, linear regression models and models fo...

HE RECENT EMPHASIS on disorders of acid-base metabolism has necessitated a reappraisal of those urinary constituents which represent elimination of acid or base. The conventional expression for urinary acid excretion (“net acid excretion”) is titratable acid bicarbonate + ammonia (T.A. CO2 + NH4 ) (1). It has been the contention of this laboratory that acid excretion is more inclusively represe...

Dye sensitizers can significantly affect power conversion efficiency of dye-sensitized solar cells (DSSCs). Porphyrin-based dyes are promising sensitizers due to their performances in DSSCs. Here, based upon a N-fused carbazole-zinc porphyrin-free-base porphyrin triad containing an ethynyl-linkage (coded as DTBC), the novel porphyrin dyes named DTBC-MP and DTBC-TP were designed by varying the p...

We describe a transient-state polarized fluorescence-based method for detecting nucleic acids. An active ester of the phthalocyanine dye La Jolla Blue was coupled to an oligonucleotide containing an amino group at its 5' end, and the conjugate was purified by HPLC chromatography. We monitored the hybridization characteristics of the conjugate with complementary oligonucleotides and RNA as targe...

There are two common methodologies to verify signatures: the functional approach and the parametric approach. This paper presents a new approach for dynamic handwritten signature verification (HSV) using the Neural Network with verification by the Conjugate Gradient Neural Network (NN). It is yet another avenue in the approach to HSV that is found to produce excellent results when compared with...

The generalization of p-frame in Banach spaces is considered in this paper. The concepts of an $tilde{X}$-frame and a system conjugate to $tilde{X}$-frame were introduced. Analogues of the results on the existence of conjugate system were obtained. The stability of $tilde{X}$-frame having a conjugate system is studied.