The high level of DNA polymorphism at the mating-type loci of mushroom fungi has made the cloning of mating-type genes difficult. As an alternative to strategies that employ sequence conservation, an approach utilizing conserved gene order could facilitate the cloning of A mating-type genes from mushroom fungi. It has been shown that a gene encoding a mitochondrial intermediate peptidase (MIP) ...

We have previously characterized a gene coding for the secreted-salivary-gland-protein 11A1 (SSGP-11A1) from the Hessian fly, Mayetiola destructor (Say) (Diptera Cecidomyiidae). Here we report the cloning and characterization of three new genes coding for proteins designated SSGP-11B1, SSGP-11C1, and SSGP-11C2, and their relationship with the SSGP-11A1-encoding gene. Based on their structural c...

We report the identification, characterization, and cloning of a novel Drosophila circadian rhythm gene, dClock. The mutant, initially called Jrk, manifests dominant effects: heterozygous flies have a period alteration and half are arrhythmic, while homozygous flies are uniformly arrhythmic. Furthermore, these flies express low levels of the two clock proteins, PERIOD (PER) and TIMELESS (TIM), ...

This talk consists of two parts. In the first, the present experimental bounds on the anomalous couplings of the gauge bosons, based mainly on the LEP and Tevatron experiments, are reviewed. In the second part, the theorem of helicity conservation (HC) is presented, which should be valid in either the Standard Model (SM) or MSSM, for any two-body process at high energies and fixed angles. The e...

background: the homologous recombination (hr) is one of the conventional cloning methods for the production of recombinant dna. it is a quick method for in vivo dna cloning without using the high cost restriction enzymes. a few modifications in the cloning procedure can increase the efficiency of this method. m aterials and methods: in this study, effect of heating on the rate of the igg1 heavy...

Over a decade passed between Friedman's discovery of the mammalian leptin gene (1) and its cloning in fish (2) and amphibians (3). Since 2005, the concept of gene synteny conservation (vs. gene sequence homology) was instrumental in identifying leptin genes in dozens of species, and we now have leptin genes from all major classes of vertebrates. This database of LEP (leptin), LEPR (leptin recep...

The Xist locus plays a central role in the regulation of X chromosome inactivation in mammals, although its exact mode of action remains to be elucidated. Evolutionary studies are important in identifying conserved genomic regions and defining their possible function. Here we report cloning, sequence analysis, and detailed characterization of the Xist gene from four closely related species of c...