Fixed chromosomes from human amniotic fluid cells and peripheral blood lymphocytes were digested in situ with exonuclease III and the single stranded DNA obtained was used as template for an in situ random primer extension. Under these conditions an R banding pattern, more evident in lymphocytes than in amniocytes, was obtained. Nevertheless, constitutive heterochromatin of chromosomes 1, 16, Y...

Karyotype, C-banding and nucleolar organizer regions (NORs) characteristics of nine Rana ridibunda samples collected from Konya (Meram, Beyşehir, Hadim) province were examined. The diploid number of chromosomes (2n) and the fundamental number of chromosome arms (FN) were determined as 26 and 52, respectively. All of the chromosomes of this species have centromeric constitutive heterochromatin (...

three subspecies of mus musculus have been recognized in iran so far. the house mouse (genus0t 0t2tmus2t, species0t 0t2tmus musculus2t) are recognized for their highly conserved morphology and chromosomal structure, but some chromosomal characters offer accurate taxonomic markers in this species that has been shown any unambiguous diagnostic morphological traits. among the chromosomal character...

Constitutive heterochromatin comprises a substantial fraction of the eukaryotic genomes and is mainly composed of tandemly arrayed satellite DNAs (satDNA). These repetitive sequences represent a very dynamic and fast evolving component of genomes. In the present work we report the isolation of Cricetus cricetus (CCR, Cricetidae, Rodentia) centromeric repetitive sequences from chromosome 4 (CCR4...

The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridization (FISH) with 18S and 5S ribosoma...

The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38-46), Dicerca aenea validiuscula Semenov, 1895 - 2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 - 2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as we...

Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding pattern, indicating that the heterochromatin of this species is abundant i...

The family Phyllostomidae belongs to the most abundant and diverse group of bats in the Neotropics with more morphological traits variation at the family level than any other group within mammals. In this work, we present data of chromosome banding (G, C and Ag-NOR) and Fluorescence In Situ Hybridization (FISH) for representatives of Rhinophylla pumilio Peters, 1865 collected in four states of ...

The karyotype and C-banding pattern of domestic Greylag geese Anser anser anser populations collected from five localities in El Minia, Egypt, that have either whitish grey or white feather color patterns were described. All populations have a diploid number of 2n= 80 chromosomes. Of the 80 chromosomes, 10 pairs, including ZW chromosomes, were macrochromosomes and the remaining 30 pairs were mi...

Phyllotisxanthopygus (Waterhouse, 1837) is an Andean rodent endemic to South America. Despite its wide geographical distribution in Argentina, few individuals have been studied on the cytogenetic level and only through conventional staining. In this work, chromosome characterization of Argentine samples of this species was performed using solid staining, C-banding and base-specific fluorochrome...