The recently introduced Clonetics human corneal epithelium (cHCE) cell line is considered a promising in vitro permeability model, replacing excised animal cornea to predict corneal permeability of topically administered compounds. The purpose of this study was to further characterize cHCE as a corneal permeability model from both drug metabolism and transport aspects. First, good correlation w...

Isotonic, neutral buffered solutions of benzalkonium chloride or chlorhexidine digluconate were applied topically to one eye of rabbits or human subjects. Contralateral control eyes received phosphate buffered saline as placebo. One-half hour later, the tear film of both eyes was loaded with nonpreserved sodium fluorescein. Anterior chamber fluorescence levels were measured at 1 hr intervals to...

Page 57 § 1.1.2.: it is mentioned that some materials can cause serious corneal injury without appearing to change opacity or permeability immediately (e.g. mustard gas). Is this a specific observation for mustard gas or are other examples (agents) known which can induce damage without changes in opacity and/or permeability? Injury (cell death) without any change in opacity and/or permeability ...

PURPOSE To study the effects of long-term contact lens wear on morphologic and physiologic properties of corneal endothelial cells. METHODS The endothelial permeability to fluorescein and the rate of corneal deswelling from hypoxia-induced edema were measured in 20 long-term (mean, 17+/-9 years; range, 5-33 years) contact lens wearers and 20 age-matched control subjects. From these data, the ...

PURPOSE The main purpose of this study was to develop a cell culture model of immortalized epithelium from the human cornea for drug permeability testing. METHODS Immortalized human corneal epithelial (HCE) cells were grown on filters, with various filter materials and coating procedures. In the optimal case, HCE cells were grown on polyester filters coated with rat tail collagen gel containi...

PURPOSE To assess the effects of diabetes mellitus on corneal structure and function. METHODS The authors measured endothelial permeability to fluorescein and corneal deswelling for 7.5 hours after 2 hours of hypoxic contact lens wear in 20 patients with diabetes who had nonproliferative retinopathy and 21 age-matched control subjects. Central corneal endothelial photographs were also taken. ...

AIMS The corneal epithelial permeability during extended wear of disposable contact lenses was compared with that during daily wear of soft contact lenses. The study was performed to verify whether the extended wear of disposable contact lenses would result in a higher permeability value than the daily wear of soft contact lenses. A higher permeability makes the cornea more vulnerable for bacte...

PURPOSE To assess corneal structure and the effects of acute hyperglycemia on corneal function in subjects with type 1 diabetes. METHODS Twenty-one diabetic and 21 nondiabetic volunteers of similar age were recruited. Baseline measurements of intraocular pressure (IOP), corneal thickness (CT), corneal autofluorescence (CAF), corneal sensitivity (CST), central and temporal endothelial cell den...