Human papillomavirus (HPV) can be detected by DNA amplification from clinical samples. The aim of the present study was to compare the HPV status in both cervical scrape and biopsy specimens obtained from 174 patients, using the recently developed broad spectrum SPF(10) PCR-LiPA method. The detection rate of HPV in these materials was determined and the spectrum of HPV genotypes was compared. C...

Skin scrapings should be the most common diagnostic test performed in veterinary dermatology. A dull scalpel blade or similar instrument is moistened with mineral oil and used to scrape away some of the epidermis, in which may reside a number of different parasites. There are 2 types of skin scrapes that should be performed. A superficial skin scrape to rule out ecotoparasites of the superficia...

An experiment was conducted to compare the whole carcass rinse method (WCR) versus scraping the breast skin or back skin for enumeration of total coliforms and E. coli. In each of two replicate trials, four pre-chill broiler carcasses were collected from two different commercial processing plants. WCR was conducted on each carcass using 400 mL 1% peptone and 1 min of shaking. After the WCR, a b...

BACKGROUND Oncogenicity of human papillomavirus (HPV) DNA in premalignant and malignant uterine cervical diseases is mainly induced by E6/E7 open reading frame (ORF). The presence of an oncogenic HPV DNA may be a diagnostic marker for the detection of cytologically negative smears. AIMS To evaluate an original polymerase chain reaction enzyme immunoassay (PCR-EIA) for the detection and typing...

BACKGROUND & OBJECTIVE Infective keratitis is rare in the absence of predisposing factors. The pattern of risk factors predisposing to infective keratitis varies with geographical regions and also influences the type of infection that occurs. The present study was aimed to identify the specific risk factors that predispose the infective keratitis (non viral) and to determine the association bet...

AIMS Gene promoter hypermethylation is recognised as an essential early step in carcinogenesis, indicating important application areas for DNA methylation analysis in early cancer detection. The current study was set out to assess the performance of CADM1, MAL and miR124-2 methylation analysis in cervical scrapes for detection of cervical and endometrial cancer. METHODS A series of cervical s...

Host contact structure affects pathogen transmission in host populations, but many measures of do not distinguish contacts that are relevant to from those not. Scrapes sites for chemical communication by white-tailed deer (Odocoileus virginianus) during the breeding season and potential prions, causative agent chronic wasting disease (CWD). Scrape-related behaviors vary their probability transm...

normal 0 false false false en-us x-none ar-sa background : the aim of this study was to determine the correlation between corneal hysteresis (ch) and the corneal resistance factor (crf), which are both novel methods of analyzing ocular rigidity/elasticity, and various corneal characteristics, mainly corneal volume in normal subjects. methods : this cross-sectional study included 500 normal eyes...

This study aimed to assess the role of specific human papillomavirus type 16 (HPV-16) variants, in combination with p53 codon 72 polymorphism genotypes, in cervical carcinogenesis. An initial sequence analysis of HPV-16 long control, E6 and E7 regions of 53 well-defined cervical samples containing HPV-16 revealed that a T to G transition at nucleotide position 350 within the E6 open reading fra...