background: prostate cancer is one of the leading causes of cancer deaths among men. new gene expressed in prostate (ngep), is a prostate-specific gene expressed only in normal prostate and prostate cancer tissue. because of its selective expression in prostate cancer cell surface, ngep is a potential immunotherapeutic target. to target the ngep in prostate cancer, it is essential to investigat...

Examination of interactions between a transmembrane protein and a soluble protein by pull-down or immunoprecipitation assays can be tricky and complicated due to the detergent extraction of membrane proteins during the lysate preparation step. The choice and concentration of detergents must be determined empirically and the procedure can be burdensome. Here, we describe a simplified binding ass...

Polr(A) pBCO is designed for assaying promoter activity of sequences cloned upstream of the CAT gene, and was obtained by cloning a Bam HI Hind III fragment from pSVOCAT into the Bam HI and Hind III sites of Bluescript KS+. Plasmids pBCTKp-1 and -2, and pBCSVp-1 and -2 are designed for assaying enhancer or silencer activity, and were obtained by cloning the fragments indicated from pMSV.TK.CAT ...

Previous studies have suggested that HDL retroendocytosis may play a role in scavenger receptor class B type I (SR-BI)-dependent selective lipid uptake in a cell-specific manner. To investigate this possibility, we developed methods to quantitatively measure HDL uptake and resecretion in fibroblast (COS-7) and hepatocyte (HepG2) cells expressing exogenous SR-BI. Approximately 17% and 24% of HDL...

Background The radiosensitivity of colon cancer cells can be regulated by noncoding RNAs. Objectives In this study, the lncRNA antisense non-coding RNA in INK4 locus (ANRIL) was selected to analyze its regulatory role chitooligosaccharides (COS)-related cells. Material and methods ANRIL expression cell lines examined using real-time quantitative polymerase chain reaction (RT-qPCR), based on whi...

shiga toxins (stxs) are bacterial virulence factors produced by shigella dysenteriae serotype 1 and escherichia coli strains. stxs are critical factors for the development of diseases such as severe bloody diarrhea and hemolytic uremic syndrome. additionally, stxs trigger the secretion of pro- inflammatory cytokines and chemokines, particularly in monocytes or macrophages. the inflammatory cyto...

Fusion of the lentogenic strain "Clone 30" of Newcastle disease virus (NDV) with the cell line COS-7 has been studied. Fusion was monitored using the octadecylrhodamine B chloride dequenching assay [Hoekstra, D., de Boer, T., Klappe, K. and Wilschut, J. (1984). Biochemistry 23, 5675-5681]. In the present work, fusion of NDV with COS-7 cells was found to occur in a time- and temperature-dependen...

background: the organochlorine ddt has estrogenic activity but the mechanism underlying the estrogenic activity of this pesticide remains unclear. in the present investigation here, we studied the transcriptional effects of a synthetic organochlorine pesticide o,p’-ddt [1.1.1.-trichloro-2-(o-chlorophenyl)-2-p-chloriphenyl ethane] and its metabolite p,p'-dde (2-2-bis(4/chlorophenyl)-1-1-dic...