In a grove in north-eastern Morocco 2020, severe root rot and crown symptoms were noticed on olive trees (Olea europaea). Pythium schmitthenneri was identified as the pathogen based conidia morphology cytochrome oxidase subunit II (COXII) gene sequence analysis. Koch's postulates verified by re-isolation of fungus P. from artificially inoculated seedlings after pathogenicity test. This is first...

Although the physiological role of plant mitochondria is thought to vary in different tissues at progressive stages of development, there has been little documentation that the complement of mitochondrial proteins is altered in different plant organs. Because the phenomenon of cytoplasmic male sterility suggests an unusual function for mitochondria in floral buds, we examined the tissue-specifi...

the possibility of occurrence of some rearrangements inside and or around coxii and atp6 genes and their relationship with male sterility in rice lines having wild abortive (wa)  cytoplasm was studied. two sterile (ir58025a and ir62829a) and two maintainer lines (ir58025b and ir62829b) were used. radioactive southern blotting was employed to carry out the experiments. the hybridization of coxii...

To evaluate the effects of dietary hemicellulose from corn on growth and metabolic measures, female pigs (n = 48; initial BW 30.8 kg) were fed diets containing 0 to 38.6% solvent-extracted corn germ meal for 28 d. Increasing the hemicellulose level had no impact on ADG or ADFI, but resulted in a quadratic response (P < 0.03) on G:F. To investigate physiological changes that occur with increased...

The biosynthesis of methylamine dehydrogenase (MADH) from Paracoccus denitrificans requires four genes in addition to those that encode the two structural protein subunits, mauB and mauA. The accessory gene products appear to be required for proper export of the protein to the periplasm, synthesis of the tryptophan tryptophylquinone (TTQ) prosthetic group, and formation of several structural di...

Defects in RNA-binding proteins have been implicated in human genetic disorders. However, efforts in understanding the functions of these proteins have been hampered by the inability to obtain their mRNA substrates. To identify cognate cellular mRNAs associated with an RNA-binding protein, we devised a strategy termed isolation of specific nucleic acids associated with proteins (SNAAP). The SNA...

Hepatocellular carcinoma (HCC) cells exhibit multidrug resistance (MDR), but the underlying mechanisms remain unclear. Cancer cells that overexpress telomerase are resistant to chemotherapeutic drugs. This study aimed to determine the effects of mitochondrial translocation of telomerase on MDR in HCC cells. HepG2 cells were transfected with negative plasmid and PTPN11 (Shp-2) short hairpin RNA ...