The enzymatic method for the determination of serum creatinine is accepted as one of the standard method in a clinical laboratory. The enzymatic method for the determination of serum creatinine was optimized for use with Merilyzer AutoQuant-400 auto analyzer and its performance characteristics were practically compared with Jaffe’s Kinetic. Effects of some interfering substances like Serum bili...

Errors of more than 10 mg/L in measured serum creatinine concentrations were encountered for an azotemic patient who was given lactulose orally. The magnitude of the apparent error fluctuated with the dose of lactulose. Additions of lactulose to serum produced a linear increase in the creatinine measured by each of two automated methods that involve use of the alkaline picrate (Jaffé) reaction....

The determination of creatinine by HPLC was performed by direct injection of serum onto the column, and after ultrafiltration of the sample, and the results were compared. A modified weak cation exchange column was used for HPLC. This eliminates the protein displacement effect and the Gibbs-Donnan effect, thus permitting a rapid direct analysis. Three of the four ultrafiltration methods gave al...

Objective(s) Comparison of prooxidant-antioxidant balance (PAB) assay with crocin assay. Materials and Methods Twenty eight serum samples were chosen, PAB and the total antioxidant capacity were measured by PAB assay and crocin, respectively, and the correlation of both assays, along with their correlation with other clinical and biochemical parameters, were determined. Results A significa...

Levels of 5-fluorocytosine in blood were rapidly calculated by determining the amount of competition in the creatinine iminohydrolase (creatinine deiminase; EC 3.5.4.21) assay for creatinine with the Kodak Ektachem system (Eastman Kodak Co., Rochester, N.Y.). The correlation with bioassay values was extremely high (r = 0.982). Standards and samples were highly stable over time.

Bilirubin causes underestimation of serum creatinine in the Jaffé alkaline picrate assay. We report an approach for preventing bilirubin interference by pretreating serum samples with peroxidase and H2O2. The dissociation of bilirubin from albumin and its subsequent oxidation markedly reduces the bilirubin interference and enables accurate determination of creatinine concentrations by the Jaffé...

A patient who ingested a methanol/nitromethane mixture (model airplane fuel) showed an apparent plasma creatinine concentration of 8.0 mmol/L by the Jaffé reaction when the actual creatinine concentration, as measured by a specific enzymatic method, was 0.09 mmol/L. This effect was due to nitromethane in the plasma. Interference by nitromethane with the determination of creatinine by reaction w...

Estimation of kidney function by measuring serum creatinine is one the commonest laboratory tests conducted in clinical practice. Enzymatic methods are often used to measure serum creatinine. Clinicians should be aware of the limitations of these methods, such as test interference with paraproteins.We present a case of falsely elevated serum creatinine in a patient referred for renal biopsy. Th...