Background. Bioactive microbial metabolites provide a successful source of novel compounds with pharmaceutical potentials. The bacterium Pseudomonas sp. In5 is a biocontrol strain isolated from a plant disease suppressive soil in Greenland, which produces two antimicrobial nonribosomal peptides (NRPs), nunapeptin and nunamycin. Methods. In this study, we used in vitro antimicrobial and anticanc...

We report the effect of Stryphnodendron adstringens on the trypanosomatid Herpetomonas samuelpessoai. The parasites were grown at 28 degrees C in a chemically defined medium containing crude extract and fractions at concentrations from 100 to 5000 microg/ml obtained from S. adstringens. Concentrations of 500, 1000, 2500, and 5000 microg/ml both crude extract and semi-purified fraction progressi...

This study was conducted in order to tenderise muscle foods (pork, beef and chicken) by using crude enzyme extract from Calotropis procera latex. Chunks of knuckle muscle from pork and beef as well as of breast muscle from chicken were marinated with distiled water (control) and 0.05%, 0.1%, 0.2%, 0.3% and 0.5% (w/w) of crude enzyme extract powder for 60 min at 4°C. The marinated samples were t...

A lentil-lectin purified glycoprotein (LL-Gp) and a crude saline extract of Taenia solium metacestodes were compared for the immunodiagnosis of human cysticercosis by immunoblotting. The LL-Gp preparation was 95% sensitive for antibodies against a range of seven antigens with molecular masses of 50 to 13 kDa, whereas the sensitivity of the crude saline extract for the detection of antibodies ag...

Crocodile blood extracts; serum, plasma, hemoglobin, and crude leukocyte extract were prepared from whole blood of crocodiles (Crocodylus siamensis) by differential sedimentation. In this study, crocodile blood extracts possessed considerable cytotoxic activity. It was evident that, the serum, plasma, hemoglobin, and crude leukocyte extract have not significant cytotoxic potentials by the yello...

objective: the present study was an attempt to study total phenolic content and antioxidant property of the crude ethanolic extract of the roots of azadirachta indica (a. indica). materials and methods: to evaluate the antioxidant properties of the crude extract, some complementary test systems, namely dpph free radical scavenging assay, reducing power assay, and ferrous ion chelating ability a...

To study the anti-diabetic effects of the crude leaves extract and fractions of Thymus schimperi Ronniger in normal and steptozotocin induced diabetic mice. The crude extract and the fractions were screened for antidiabetic activities in streptozotocin induced diabetic mice. A normoglycemic mice model and oral glucose tolerance test were also undertaken to assess the hypoglycemic and antihyperg...

results in vitro analysis showed that the greater the concentration of the extract, the greater the cytotoxicity. conclusions the crude extract of p. australis might be cytotoxic at high concentrations as evaluated against the cell line. materials and methods the mtt cytotoxicity assay was performed on a 3t3 mouse fibroblast cell line to measure the in vitro cytotoxic activity of the crude form...

The crude extract of Ampelopsis cantoniensis induced apoptosis in human promyelocytic leukemia HL-60 cells and this induction was investigated by flow cytometric analysis, DNA gel electrophoresis and poly (ADP-ribose) fluorescence staining. The results demonstrated that this extract induced dose-dependent cytotoxicity and apoptosis. The level of active caspase-3 was increased after treatment wi...

this study was designed to examine bioactive compounds of the thyme-enriched corn oil and to determine the antioxidant activity of its methanolic extract. volatile compounds composition was investigated by gas chromatography-mass spectrometry (gc-ms), whereas phenolic compounds analysis was performed by reversed-phase high-performance liquid chromatography (rp-hplc). antioxidant activities of t...