background: spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. the routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. objective: to compare between the application of slow cryopreservation and vitri...

BACKGROUND Understanding how leukocytes in the cervicovaginal and colorectal mucosae respond to pathogens, and how medical interventions affect these responses, is important for developing better tools to prevent HIV and other sexually transmitted infections. An effective cryopreservation protocol for these cells following their isolation will make studying them more feasible. METHODS AND FIN...

BACKGROUND Successful long-term preservation of adipose tissues may have an important impact on future clinical application of autologous fat transplantation. Our group has recently developed an optimal cryopreservation method for possible long-term preservation of adipose aspirates. OBJECTIVE The purpose of this study was to evaluate the fate of previously cryopreserved adipose aspirates aft...

To determine the best and simplest method for cryopreservation of pig hepatocytes, we compared immediate cryopreservation with cryopreservation after short-term culture. Suckling pig hepatocytes were isolated by a modified 2-step in situ collagenase perfusion method, suspended in serum-free medium, and preserved for 10 da by two cryopreservation methods. Serial measurements were made of cell vi...

Cryopreservation of embryos and gametes has become an indispensible service for infertility treatment. Vitrification is now a prevalent technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology. Fertility preservation in female patients has become a very topical issue. Various malignant and nonmalignant diseases have...

With the increase in survivorship following cancer for women in their reproductive years, as well as an increase in survivorship with childhood cancers, there is a demand for perfecting current fertility preservation methods and generating new options for patients who are unable to pursue the conventional course of fertility treatments. Cryopreservation using a slow-cooling method for embryos i...

OBJECTIVE To review historical and contemporary advances in oocyte-cryopreservation techniques and outcomes. DESIGN Publications related to oocyte cryopreservation were identified through MEDLINE and other bibliographic databases. CONCLUSION(S) Oocyte cryopreservation can be used as an adjunct to conventional IVF and as an option for fertile women to electively cryopreserve their gametes. R...

Backgroud: Oocyte cryopreservation is critical in assisted reproduction techniques. However, the effect of cryopreservation on the oocyte has not been widely explored yet. Currently a thorough understanding of oocyte cryobiology is urgently required due to its far lag behind the forces propelling the clinical application of oocyte cryopreservation. Methods: In this study, we used two different ...

BACKGROUND Sperm cryopreservation remains the only clinically feasible option to preserve male fertility. The quality of counselling provided by the treating physicians and the cost of sperm cryopreservation can both influence a patient's decision about whether to preserve sperm. On 5 August 2010, the Quebec government introduced provincial coverage of assisted reproductive technologies, with s...

Cellular therapy with stem and immune cells has demonstrated significant success both in clinical treatments and the industrial market. Cryopreservation is a necessary and essential component of cellular therapy. In this chapter, first of all, some basic theories of cryoinjury and techniques in cryopreservation are reviewed. Then it focuses on the progress of cryopreservation of stem cells and ...