Results with two ultramicro methods for plasma uric acid analysis with uricase are described: a crystal violet (gentian violet) method, modified from an automated procedure by Gochman and Schmitz [C/in. Chem. 17, 1154 (1971)] and adapted to manual analysis, and a polarographic oxygen-sensing system (Beckman Instruments,Inc.).The coefficientof variation (CV) for the crystal violet method varied ...

In this work, we used a simple and totally green method for synthesizing silver nanoparticles using kappa-carrageenan as reducing and stabilizing agent. The beads were prepared in aqueous medium by microwave heating, and then followed by cross-linking with K+ cations without using any additional toxic and expensive chemical agents. The preparation method of the carrageenan-based beads is easy, ...

The application of Leuco Crystal Violet (LCV) for the enhancement of footwear and fingerprint impression evidence was first utilized in the FBI Laboratory in late 1993, after learning from Mr. John F. Fischer (1) of the formulation of this reagent. Mr. Fischer subsequently made a presentation on this topic at the International Symposium on Footwear and Tire Tread Impression Evidence at the FBI ...

Effluent of various industries contain number of inhibitory substances which are continuously added in sea via river and results in water pollution. There is need to degrade the organic content to abate water pollution. In the present study, actinomycetes were isolated on glycerol aspargine agar from marine sample of Calangut beach, Goa. The isolates were tested for their potential to tolerate ...

The effect of oxidative stress exerted by 0.03% crystal violet, 0.3% bile salt, 4.0% NaCl and 8% ethanol on the survival and production of virulence factors among Acinetobacter haemolyticus isolates obtained from wastewater and river water samples was investigated. Though generally there was insignificant lethal effect against all the isolates, crystal violet exerted the highest lethal effect f...

Ninety-nine selected compounds and eleven antibiotic-producing organisms were tested for antiphage activity and host toxicity. A paper disc-agar diffusion method was used for primary screening and quantitative methods were employed for confirmatory investigation. Most of the agents tested, although previously reported as inhibitory to one or more other virus-host systems, did not selectively pr...

Plasmid pGNB1 was isolated from bacteria residing in the activated sludge compartment of a wastewater treatment plant by using a transformation-based approach. This 60-kb plasmid confers resistance to the triphenylmethane dye crystal violet and enables its host bacterium to decolorize crystal violet. Partial sequencing of pGNB1 revealed that its backbone is very similar to that of previously se...

Several papers have been published (Kennedy and Barbaro, 1953; Barbaro and Kennedy, 1954; Bartholomew and Finkelstein, 1954; Kennedy and Woodhour, 1956; Barbaro et al., 1956) on methods of comparing degrees of gram-positiveness and the influence of certain factors on the quantity of crystal violet retained by the microorganisms. These papers compare the degrees of gram-positiveness by measuring...

A new consortium of four bacterial isolates (Agrobacterium radiobacter; Bacillus spp.; Sphingomonas paucimobilis, and Aeromonas hydrophila)-(CM-4) was used to degrade and to decolorize triphenylmethane dyes. All bacteria were isolated from activated sludge extracted from a wastewater treatment station of a dyeing industry plant. Individual bacterial isolates exhibited a remarkable color-removal...