A multiplex Taqman-based real-time reverse transcription (RT) polymerase chain reaction (PCR) assay was developed to identify potential severe strains of Citrus tristeza virus (CTV) and separate genotypes that react with the monoclonal antibody MCA13. Three strain-specific probes were developed using intergene sequences between the major and minor coat protein genes (CPi) in a multiplex reactio...

PURPOSE Dose coverage is crucial for successful treatment in mono-brachytherapy. Since few and very high dose fractions are used, there is an important balance between dwell positioning outside the clinical target volume (CTV) and possible damage on adjacent normal tissue. The purpose of this study was to evaluate the possibility of having dwell positions close to the CTV surface, while maintai...

PURPOSE To analyze the distribution of functional liver volume (FLV) in the margin volume (MV) surrounding hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT) before radiation therapy (RT) and to verify the safety of single photon emission computed tomography-based three-dimensional conformal radiotherapy (SPECT-B3DCRT) by exploring the relation of FLV in MV to radiation-induc...

The 23 kDa protein (p23) coded by the 3 -terminal gene of Citrus tristeza virus (CTV), is a RNA-binding protein that contains a motif rich in cysteine and histidine residues in the core of a putative zinc-finger domain. Transgenic Mexican lime plants were generated carrying a p23 transgene, or a truncated version thereof, under the control of the Cauliflower mosaic virus (CaMV) 35S promoter. Ec...

Abstract Hop stunt viroid (HSVd) and Citrus tristeza virus (CTV) are the causal agents of most important diseases of citrus. Previously we reported a novel variant of HSVd that constantly associated with the yellow corky vein disease. Here, 19 individual citrus leaves with yellow corky vein symptoms were sampled for CTV by ELISA. In this study, most symptomatic samples were found to be inf...

PURPOSE We used high-dose cyclophosphamide plus topotecan/vincristine (CTV) or irinotecan (C/I) in patients with resistant neuroblastoma. The aim was to use a regimen with little risk to major organs to (a) achieve or consolidate remission in heavily treated patients and to (b) induce an immunological state conducive to passive immunotherapy with the murine 3F8 antibody. EXPERIMENTAL DESIGN C...

Background: This study evaluates dosimetric and spatial variations in inter–fraction applicator positioning in high dose rate (HDR) brachytherapy. Materials and Methods: This study includes 25 retrospective patients of carcinoma of the cervix. Each patient received 5 fractions of HDR intracavitary brachytherapy. High Risk-Clinical Target Volume (HR-CTV) were drawn on CT images. First impl...

BACKGROUND Venous thromboembolic disease is a major cause of mortality and morbidity. OBJECTIVES The aim of this study is to compare emergency physician-performed ultrasound (EPPU) of the lower extremities with CT venography (CTV) in emergency department (ED) patients undergoing workup for pulmonary embolism (PE). METHODS This was a prospective study performed at a busy academic ED. Adult p...

Citrus tristeza virus (CTV) is the causal agent of tristeza disease, which is one of the most devastating diseases of citrus crops worldwide. This paper describes a method for the rapid detection and genotyping of naturally spreading CTV isolates. This method uses ELISA or dot-blot immunological tests to detect trees infected with CTV. The reaction wells or membrane spots for which there is a p...

BAR-JOSEPH, M., S. M. GARNSEY, D. GONSALVES, M. MOSCOVITZ, D. E. PURCIFULL, M. F. CLARK, and G. LOEBENSTEIN. 1979. The use of enzyme-linked immunosorbent assay for detection of citrus tristeza virus. Phytopathology 69:190-194. An enzyme-linked immunosorbent assay (ELISA) test was used to seedling yellows isolates of CTV from Israel and from Florida. Isolates that identify citrus tristeza virus ...