The cyclic cystine knot motif, as defined by the cyclotide peptide family, is an attractive scaffold for protein engineering. To date, however, the utilisation of this scaffold has been limited by the inability to synthesise members of the most diverse and biologically active subfamily, the bracelet cyclotides. This study describes the synthesis and first direct oxidative folding of a bracelet ...

A selective N→S acyl transfer reaction facilitates semi-synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N-terminal cysteine and a thiol-labile C-terminal Gly-Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements.

Park, S. 2016. Cyclotides evolve. Studies on their natural distribution, structural diversity, and activity. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy 218. 71 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9604-3. The cyclotides are a family of naturally occurring peptides characterized by cyclic cystine knot (CCK) structural motif, whic...

Antimicrobial peptides (AMPs) are small defense proteins present in various organisms. Major groups of AMPs include beta-barrelin, hevein, knottin, lipid transfer protein (LTP), thionin, defensin, snakin, and cyclotide. Most plant AMPs involve host plant resistance to pathogens such as fungi, viruses, and bacteria, whereas a few plant AMPs from the cyclotide family carry insecticidal functions....

BACKGROUND Oldenlandia affinis, commonly called 'kalata-kalata', a versatile plant used locally to treat malaria fever in some parts of sub-Saharan Africa was investigated for anti-plasmodial and anti-inflammatory activities. OBJECTIVE The study was designed to evaluate the antiplasmodial as well as anti-inflammatory activities of whole extract and cyclotide-rich fraction of Oldenlandia affin...

background :    searching for the potent natural antibiotics will improve the treatment of most infectious diseases. cyclotides are potent active peptides derived from some plant families like violaceae. viola tricolor (the pansy) has been found to have numerous cyclotides. nowadays cyclotides attract more attention for their antibacterial activities.  the current research studied the antimicro...

Cyclotides are peptides from plants of the Rubiaceae and Violaceae families that have the unusual characteristic of a macrocylic backbone. They are further characterized by their incorporation of a cystine knot in which two disulfides, along with the intervening backbone residues, form a ring through which a third disulfide is threaded. The cyclotides have been found in every Violaceae species ...