Cystatin C is a human cysteine proteinase inhibitor present in extracellular fluids. Cystatin C and a tripeptide derivative (Z-LVG-CHN2) that mimics its proteinase-binding center, were tested for possible antiviral activity against herpes simplex virus type 1 (HSV) and poliovirus type 1. Both recombinant cystatin C and Z-LVG-CHN2 displayed strong inhibitory effects on HSV replication, whereas n...

Feeding bioassay results established that the soybean cysteine proteinase inhibitor N (soyacystatin N, scN) substantially inhibits growth and development of western corn rootworm (WCR), by attenuating digestive proteolysis [Zhao, Y. et al. (1996) Plant Physiol. 111, 1299-1306]. Recombinant scN was more inhibitory than the potent and broad specificity cysteine proteinase inhibitor E-64. WCR dige...

Thougha numberof proteinaceous cysteine proteinase inhibitors (cystatins) of animal origin have long been studied in detail,1* little is well-defined about plant cystatins except for oryzacystatins from rice seeds.2'3) Preceding the studies on oryzacystatins, however, we purified a cysteine proteinase inhibitor from corn.4'5) In this study we carried out amino acid analysis and kinetic studies ...

purpose: evaluation of humoral and cellular immune responses of naturally infected dogs against type i (rcpb) (recombinant cycsteine proteinase b), and ii (rcpa) (recombinant cycsteine proteinase a) recombinant cysteine proteinases and c-terminal extension (cte) of leishmania infantum (l. infantum). materials and methods: in this study, fourteen infected dogs (7 with symptoms, 7 asymptomatics)...

We have used a specific inhibitor of the malarial aspartic proteinase plasmepsin I and a nonspecific cysteine proteinase inhibitor to investigate the importance of hemoglobin degradation in the mechanism of action of chloroquine, amodiaquine, quinine, mefloquine (MQ), halofantrine, and primaquine. Both proteinase inhibitors antagonized the antiparasitic activity of all drugs tested with the exc...

Interactions between connective tissue substrates and proteinases localized to the surface of cancer cells are implicated in cancer invasion. In this report we have compared the enrichment of collagen and gelatin degrading activities and cysteine proteinase(s) in well-characterized (enzyme markers and electron microscopy) subcellular membrane fractions isolated from human small cell lung cancer...

The major proteinase activity in extracts of larval midguts from the southern corn rootworm (SCR), Diabrotica undecimpunctata howardi, was identified as a cysteine proteinase that prefers substrates containing an arginine residue in the P1 position. Gelatin-zymogram analysis of the midgut proteinases indicated that the artificial diet-fed SCR, corn root-fed SCR, and root-fed western corn rootwo...