Four methods for the assay of human interferon in Vero cells were compared based on the inhibition of viral cytopathic effect (CPE) in tubes, the inhibition of CPE in microplates, the reduction of plaques, and the inhibition of quantitative hemadsorption. For inhibition of CPE, Sindbis virus, vesicular stomatitis virus, poliovirus type 2, and vaccinia virus were used for challenge. In the plaqu...

* Abbreviations: CPE = cytopathic effect, MB = methylene blue, CM = complete growth medium, CDM = chemically defined medium, BAF = bovine amniotic fluid, dil. = dilution, f = final, v.i. = vide infra, ab. = about, med = medium, TC = tissue culture. 1 E. KovÄcs, V. STÜRTZ and G. W A G N E R , Z. Naturforschg. 15 b, 116 [ I960] . 2 E. KovÄcs, G. W A G N E R and V. STÜRTZ , Z. Naturforschg. 15 b, ...

Human lung fibroblasts develop a cytopathic effect (CPE) when infected with Mycoplasma pneumoniae. This study was designed to determine the relationship between host cell metabolism and the formation of the CPE. Human lung fibroblasts were grown in different serum concentrations, plated at different densities, and grown for different periods of time to alter the metabolic activity of the cells....

The effect of the alkaloid 1-(phenyl)-N'-(4-methoxybenzylidene)- 9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero cells was assayed by inhibition of the cytopathic effect (CPE) and inhibition of plaque forming units (PFU). Both methodologies suggested that the mode of action was avoidance of infection progression in the host cell. The compound was a...

objective: titration of viruses is important to determine the quantity of virus in vaccine development, master virus seed stock preparation, viral vector studies and virus replication. in this study, we compared the ccid50% and plaque assay as a standard titration method for rotavirus (rf) and hsv-1.   methods: the ma104 and vero cells were inoculated by rf and hsv-1 in 6- and 96-well plates. f...

Background: Avipox virus considered to be a contagious disease of many types of birds wild and domestic. The current study focused on the isolation of recently common Avipox virus which include fowl pox (FP) and pigeon pox (PP) viruses in Iraq. Materials & methods: Isolation of the viruses was done via chorioallantoic membrane (CAM) route of avian embryo inoculation, also used by a chicken fibr...

Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe disease in Chinese giant salamanders, the largest extant amphibian in the world. A fish cell line, Epithelioma papulosum cyprinid (EPC), and a new amphibian cell line, Chinese giant salamander spleen cell (GSSC), were infected with ADRV and observed by light and electron microscopy. The morphological changes in...

Induction of the cytopathic effect (CPE) in cells infected with poxvirus seems ubiquitous in that it has been associated with all different strains and preparations of poxviruses, regardless of the replicating status of these viruses. The study of the mechanisms by which CPE is induced by nonreplicating poxviruses is hampered by the lack of any noncytopathic mutant strains and preparations. In ...

Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used f...