We present an EOM-CCSD based QM/MM study on the electron attachment process to cytosine, solvated in water. The microhydration studies cannot capture effect of bulk water environment and one need include large number molecules calculation get converged results. cytosine happened through a doorway mechanism, where initial is localized subsequently gets transferred by mixing electronic nuclear de...

Cytosine, G-Values, pK-Values, Absorption Spectra, Pulse Radiolysis Using the pulse radiolysis technique the OH adducts of cytosine, 5-methyl cytosine and 2'-deoxycytidine were investigated in alkaline N20 saturated aqueous solutions. Absorption spectra were recorded and their change with time was correlated with the change in conductivity. An OH induced reaction (1.4 • 108 1 • mol-1 • s -1) wa...

APOBEC3B (A3B) is a cytosine deaminase that converts to uracil in single-stranded DNA. Cytosine-to-thymine and cytosine-to-guanine base substitution mutations trinucleotide motifs (APOBEC mutational signatures) were found various cancers including lymphoid hematological malignancies such as multiple myeloma A3B has been shown be an enzymatic source of those cancers. Although the importance bein...

The gas-phase structures of alkali metal cation-cytosine complexes generated by electrospray ionization are probed via infrared multiple photon dissociation (IRMPD) action spectroscopy and theoretical calculations. IRMPD action spectra of five alkali metal cation-cytosine complexes exhibit both similar and distinctive spectral features over the range of ~1000-1900 cm(-1). The IRMPD spectra of t...

While cytosine methylation has been widely studied in extant populations, relatively few studies have analyzed methylation in ancient DNA. Most existing studies of epigenetic marks in ancient DNA have inferred patterns of methylation in highly degraded samples using post-mortem damage to cytosines as a proxy for cytosine methylation levels. However, this approach limits the inference of methyla...

The type 1 ribosome inactivating protein from Momordica balsamina (MbRIP1) has been shown to interact with purine bases, adenine and guanine of RNA/DNA. We report here the binding and structural studies of MbRIP1 with a pyrimidine base, cytosine; cytosine containing nucleoside, cytidine; and cytosine containing nucleotide, cytidine diphosphate. All three compounds bound to MbRIP1 at the active ...

DNA cytosine methylation is a widespread epigenetic mechanism in eukaryotes, and plant genomes commonly are densely methylated. Genomic methylation can be associated with functional consequences such as mutational events, genomic instability or altered gene expression, but little is known on interspecific variation in global cytosine methylation in plants. In this paper, we compare global cytos...

Survival times of C57BL/6J X DBA/2J Fj mice, inoculated with LI210 or LSI78Y ascites tumor cells, were monitored following treatment with cytosine arabinoside, L-asparaginase, and 6-azauridine, alone and in combination. Each tumor line was sensitive to cytosine arabinoside. L-Asparaginase produced cures (60-day survivors) in 20 to 30% of L5178Y hosts but was totally ineffective against L1210. 6...