Iron-sulfur clusters are prosthetic groups composed of sulfur and iron that are found in respiratory chain complexes and numerous enzymes. Iron-sulfur clusters are synthesized in a multistep process that utilizes cysteine desulfurases, scaffold proteins, chaperones, and iron donors. Assembly of iron-sulfur clusters occurs in the mitochondrial matrix of mammalian cells, but cytosolic isoforms of...

Imido alkylidene complexes of Mo and W and oxo alkylidene complexes of W that contain thiophenoxide ligands of the type S-2,3,5,6-Ph4C6H (STPP) and S-2,6-(mesityl)2C6H3 (SHMT = S-hexamethylterphenyl) have been prepared in order to compare their metathesis activity with that of the analogous phenoxide complexes. All thiolate complexes were significantly slower (up to ∼10× slower) for the metathe...

phosphatidate phosphohydrolase (pap) from cytosolic fraction of rat liver was purified to homogeneity having specific activity of 5.14 u/mg protein. an activity staining procedure was developed to determine molecular weight of the enzyme on polyacrylamide gel electrophoresis using ferguson plot. molecular weight (m.w.) of the active pap was 298 kda. sds-page analysis showed a m.w. of 47 kda for...

Boronate Building Blocks building blocks have been developed for intracellular protein delivery. Article 2202049 by Qianyi Ren, Yiyun Cheng, and Jia Lv provides a summary of boronate chemistry used in the design cytosolic delivery systems. The can address barriers existing process.

stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarifi ed how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJAand Ca 2 + -induced stomatal closure of wild type, abi1-1 , abi2-1 , ost1-2 and coi1 using a pHsensitive fl uorescent dye, BCECF-AM. ABA induc...

Adenosine has a major regulatory function in the heart and many tissues. Our previous work showed that a cytosolic (not a membrane, as previously hypothesized) 5'-nucleotidase from dog heart has the kinetic properties consistent with it being the enzyme responsible for adenosine formation from adenosine 5'-monophosphate (AMP) in response to hypoxia or ischemia. In the present study, we evaluate...