It has been shown that sequence patterns including Shine-Dalgarno sequences, start codons and those in between have effect on translational efficiencies. However relationships between these patterns and translational efficiencies are complicated and sophisticated method are required for the modeling. In this work, we constructed a model to predict translational efficiencies of genes based on se...

Sixteen single point mutations near the beginning of the lacZ gene have been isolated and their effect on lacZ expression has been measured. Five mutations were obtained that alter a potential stem-and-loop structure in the messenger RNA that masks the initiation codons. Formation of this stem-and-loop is a result of transcription of DNA sequences introduced during the cloning of the lac regula...

Youvan et al . (Cell 37, 949-957,1984) recently reported the nucleotide and deduced polypeptide sequences of the photosynthetic reaction-center, B870 antenna, and flanking polypeptides from Rhodopseudomonas capsulata . The assignments of deduced peptide sequences of unknown function were based on the existence of open reading frames in the nucleotide sequences and on the identification of likel...

Ribosomal RNA (rRNA) genes, essential to all forms of life, have been viewed as highly conserved and evolutionarily stable, partly because very little is known about their natural variations. Here, we explored large-scale variations of rRNA genes through bioinformatic analyses of available complete bacterial genomic sequences with an emphasis on formation mechanisms and biological significance....

The DNA sequences associated with 139 apparent streptomycete transcriptional start sites are compiled and compared. Of these, 29 promoters appeared to belong to a group which are similar to those recognized by eubacterial RNA polymerases containing sigma 70-like subunits. The other 110 putative promoter regions contain a wide diversity of sequences; several of these promoters have obvious seque...

The Shine-Dalgarno (SD) sequence [4] of E. coli is known to be a signal to initiate translation. The widely accepted model is that the 3’ end of 16S rRNA base-pairs with the SD sequence in the first step of ribosome binding to mRNA. However, archaebacteria have been supposed to have systems of translation different from those of eubacteria and eucaryotes. Further, some eubacteria, such as M. ge...

AIMS To develop a strategy to increase the stability of transcripts of structural genes expressed under the control of sacR, the leader region of Bacillus subtilis levansucrase gene. METHODS AND RESULTS Insertion of Shine Dalgarno like sequences in the 5'-untranslated sacR region controlling the expression of sacB. Depending on the number of stabilizing sequences inserted and the position of ...