Nowadays, a great demand for the development of novel drug delivery systems with high potential bench-to-market transition attracts scientific attention toward materials that are already approved biomedical use. Here, controlled fabrication hybrid organic inorganic mesoporous crystals is realized in physiologically relevant conditions by co-synthesis vaterite CaCO3 presence dextran (DEX) or its...

Atherosclerosis is a major cardiovascular disease. One of the side effects is restenosis. The aim of this work was to study the coating of stents by dextran derivates based polyelectrolyte's multilayer (PEM) films in order to increase endothelialization of injured arterial wall after stent implantation. Films were composed with diethylaminoethyl dextran (DEAE) as polycation and dextran sulphate...

Addition of nanomolar concentration of the phorbol ester 12-O-tetradecanoyl phorbol 13-acetate (TPA) to the human promyelocytic HL-60 leukemia cells is associated with a cessation of cellular proliferation and a subsequent differentiation into macrophage-like cells. Because the growth rate of mammalian cells is tightly coupled to the functions of the protein synthetic machinery, we have examine...

DEAE-silica gel has been shown to be an improvement over DEAE-Sephadex for the quantitative isolation of gangliosides and neutral glycosphingolipids from animal tissues or cells. Preliminary results indicated that it can also be used for protein separation. Direct comparative studies of DEAE-silica gel with DEAE-Sephadex showed preferences for the former for the following reasons: i) faster flo...

DEAE-Dextran, Dunaliella, Membrane Permeability, Photosynthesis, Polycations As a prerequisite for studies o f the intracellular distribution o f enzymes o f the glycerol cycle in Dunaliella parva, the effect o f the polycation DEAE-dextran on the permeability o f membranes for various endogenous compounds and on photosynthesis was investigated. DEAE-dextran induces an increase o f the permeabi...

The study included the extraction and purification of glucose oxidase enzyme from Penicillium chrysogenum, pure by precipitation with ammonium sulfate at a saturation rate 70% specific activity in this step reached (10.802) units/mg fold 1.276, was followed dialysis step, 13,593 48.184. Finally, ion exchange chromatography using DEAE-Cellulose column, 19.355 units/mg, 36.59, after which migrate...

Electroporation has proven to be a convenient method for transfection of many cell types. One drawback has been that it requires large amounts of DNA, typically 20 to 80 /tg per transfection (1). Here we report that adding DEAE-dextran to the electroporation media dramatically increases electroporation efficiency in mammalian cells, lowering the amount of DNA required for efficient transfection...

in this study, we present changes of peroxidase (e. c. 1.11.1. 7) activities in the root of brassica napus var. okapi during rosette stage until ripening of the fruits. peroxidase activity was determined seasonally over an eight month period. results showed that total peroxidase activity in the root was highest in mid june and the lowest activities were observed from time of winter dormancy unt...